Vijay Ajay Kumar, Willcox Mark D P
School of Optometry and Vision Science (A.K.V., M.D.P.W.), University of New South Wales, Sydney, New South Wales, Australia.
Eye Contact Lens. 2018 Nov;44 Suppl 2:S120-S126. doi: 10.1097/ICL.0000000000000425.
Contact lens cases become contaminated with microbes during use. We wished to compare the adhesion of uncommon bacterial contaminants isolated from lens cases to contact lenses with and without organic soil.
Strains of Delftia acidovorans (001), Stenotrophomonas maltophilia (002 and 006), and Achromobacter xylosoxidans (001) isolated from contact lens cases (test strains) and Pseudomonas aeruginosa (Paer1) isolated from eyes at the time of infiltrative response (control strain) were used. Bacteria were grown and resuspended in phosphate-buffered saline (PBS) or 10% organic soil (heat-killed Saccharomyces cerevisiae resuspended in complement inactivated bovine serum). Two silicone hydrogel (senofilcon A and comfilcon A) and one hydrogel lens (etafilcon A) lens materials were used. Bacteria (1.0×10 and 1.0×10 colony-forming units/mL; CFU/mL) adhered to lenses for 24 hr and the numbers of bacteria adherent to each lens type (with and without organic soil) were estimated by culture.
All the four test strains adhered in significantly greater numbers to contact lenses after incubation in inoculum prepared with organic soil compared with PBS-D. acidovorans 001 (0.7 log10 CFU; P<0.05), S. maltophilia 002 (1.7 log10 CFU; P<0.05), S. maltophilia 006 (0.9 log10 CFU; P<0.05), and A. xylosoxidans 001 (0.4 log10 CFU; P<0.05). However, the presence of organic soil did not increase adhesion of P. aeruginosa Paer1 (-0.1 log10 CFU; P>0.05). Achromobacter xylosoxidans 001 (P<0.01), D. acidovorans 001 (P<0.01), and S. maltophilia 002 (P<0.01) significantly differed in their adhesion to the three contact lens materials.
Bacteria that are commonly found in contact lens cases adhered to contact lenses in relatively high numbers in the presence of organic soil. This might indicate that a similar phenomenon occurs in the presence of tears. This may facilitate their transfer from the lens to the cornea and the production of corneal infiltrates.
隐形眼镜盒在使用过程中会被微生物污染。我们希望比较从隐形眼镜盒中分离出的罕见细菌污染物在有和没有有机污垢的情况下对隐形眼镜的粘附情况。
使用从隐形眼镜盒中分离出的嗜酸代尔夫特菌（001）、嗜麦芽窄食单胞菌（002和006）以及木糖氧化无色杆菌（001）菌株（测试菌株）和在浸润反应时从眼睛中分离出的铜绿假单胞菌（Paer1）（对照菌株）。细菌在磷酸盐缓冲盐水（PBS）或10%有机污垢（热灭活酿酒酵母重悬于补体灭活牛血清中）中培养并重悬。使用了两种硅水凝胶（senofilcon A和comfilcon A）和一种水凝胶镜片（etafilcon A）材料。细菌（1.0×10和1.0×10菌落形成单位/毫升；CFU/毫升）与镜片粘附24小时，并通过培养估计每种镜片类型（有和没有有机污垢）上粘附的细菌数量。
与PBS相比，在有机污垢制备的接种物中孵育后，所有四种测试菌株在隐形眼镜上的粘附数量显著更多。嗜酸代尔夫特菌001（0.7 log10 CFU；P<0.05）、嗜麦芽窄食单胞菌002（1.7 log10 CFU；P<0.05）、嗜麦芽窄食单胞菌006（0.9 log10 CFU；P<0.05）和木糖氧化无色杆菌001（0.4 log10 CFU；P<0.05）。然而，有机污垢的存在并未增加铜绿假单胞菌Paer1的粘附（-0.1 log10 CFU；P>0.05）。木糖氧化无色杆菌001（P<0.01）、嗜酸代尔夫特菌001（P<0.01）和嗜麦芽窄食单胞菌002（P<0.01）在对三种隐形眼镜材料的粘附方面存在显著差异。
在有机污垢存在的情况下，隐形眼镜盒中常见的细菌在隐形眼镜上的粘附数量相对较高。这可能表明在有眼泪存在的情况下会发生类似现象。这可能有助于它们从镜片转移到角膜并产生角膜浸润。
