Carneiro C C, Véras J Holanda, Góes B R Lima, Pérez C N, Chen-Chen L
Departamento de Genética, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brazil.
Instituto de Química, Universidade Federal de Goiás, Goiânia, GO, Brazil.
Braz J Biol. 2018 May;78(2):345-350. doi: 10.1590/1519-6984.166593. Epub 2017 Sep 21.
Salacia crassifolia (Mart. Ex. Schult.) G. Don. is a bush which belongs to Celastraceae family and occurs specially in Brazilian Cerrado. Its leaves, stem, seeds and fruits are popularly used for several medicinal purposes, such as antitumoral, antirheumatic, anti-inflammatory and antimicrobial. In this study, the mutagenic and antimutagenic activities of S. crassifolia stem bark fractions (hexane, ethyl acetate and hydroalcoholic) were evaluated by the Ames mutagenicity assay in Salmonella typhimurium TA98 and TA100 strains. By the obtained results, all S. crassifolia fractions did not significantly increase the number of prototrophic revertants for histidine (His+) in both S. typhimurium strains tested (p > 0.05), suggesting absence of mutagenicity. Regarding antimutagenicity, the fractions ethyl acetate and hydroalcoholic significantly decreased the number of His+ revertants colonies induced by positive control for strain TA98 (p < 0.05), demonstrating protection against mutagenicity induced by 4-nitroquinolile1-oxide, whereas the hexane fraction did not show antimutagenic effect in this strain. In the TA100 strain, all fractions of S. crassifolia protected DNA against the harmful action of sodium azide, and the hexane fraction exhibited the greatest protection in this work. Thus, it's possible conclude that the fractions of S. crassifolia tested in this study could be used in chemoprevention.
厚叶锡生藤（Salacia crassifolia (Mart. Ex. Schult.) G. Don.）是一种属于卫矛科的灌木，特别生长于巴西塞拉多地区。其叶、茎、种子和果实广泛用于多种药用目的，如抗肿瘤、抗风湿、抗炎和抗菌。在本研究中，通过鼠伤寒沙门氏菌TA98和TA100菌株的艾姆斯诱变试验评估了厚叶锡生藤茎皮提取物（己烷、乙酸乙酯和水醇提取物）的诱变和抗诱变活性。根据所得结果，在测试的两种鼠伤寒沙门氏菌菌株中，所有厚叶锡生藤提取物均未显著增加组氨酸原养型回复突变体（His+）的数量（p>0.05），表明不存在诱变活性。关于抗诱变活性，乙酸乙酯和水醇提取物显著降低了TA98菌株阳性对照诱导的His+回复突变菌落数量（p<0.05），证明对4-硝基喹啉-1-氧化物诱导的诱变具有保护作用，而己烷提取物在该菌株中未显示抗诱变作用。在TA100菌株中，厚叶锡生藤的所有提取物均保护DNA免受叠氮化钠的有害作用，且己烷提取物在本研究中表现出最大的保护作用。因此，可以得出结论，本研究中测试的厚叶锡生藤提取物可用于化学预防。
