Purim Kátia Sheylla Malta, Peretti Murilo Calvo, Fillus José, Olandoski Marcia
Discipline of Dermatology of the Medical School - Universidade Positivo (UP) - Curitiba (PR), Brazil.
Dermatology Service of theHospital das Clínicas - Universidade Federal do Paraná (HC-UFPR) - Curitiba (PR), Brazil.
An Bras Dermatol. 2017 Jul-Aug;92(4):478-483. doi: 10.1590/abd1806-4841.20175466.
: Histological and mycological changes during itraconazole use have not been totally established in chromoblastomycosis.
: To evaluate tissue modifications in chromoblastomycosis carriers under itraconazole treatment.
: A histological retrospective study of 20 cases of chromoblastomycosis seen at the university hospital at the south of Brazil, during itraconazole 400 mg daily treatment. Patients were classified into two groups: plaque or tumor lesions, and underwent periodic evaluations every four months during three years. Hematoxylin-eosin stain was used to analyze epidermal modifications, inflammatory infiltrate and fibrosis, and Fontana-Masson stain for parasite evaluation.
: Fontana-Masson stain was superior to hematoxylin-eosin stain in fungal count in the epidermis (mean difference=0.14; p<0.05). The most distinct mycosis tissue responses were registered in the dermis. Epidermal thinning, granulomatous infiltrate decrease or disappearance, fibrosis increase and quantitative/morphological changes occurred during treatment.
: Patients could not be located to have their current skin condition examined.
: Parasitic and tissue changes verified in this study can reflect the parasite-host dynamics under itraconazole action.
在着色芽生菌病中，使用伊曲康唑期间的组织学和真菌学变化尚未完全明确。
评估接受伊曲康唑治疗的着色芽生菌病携带者的组织改变。
对巴西南部大学医院收治的20例着色芽生菌病患者进行组织学回顾性研究，患者接受每日400毫克伊曲康唑治疗。患者分为两组：斑块状或肿瘤样病变组，并在三年期间每四个月进行定期评估。采用苏木精-伊红染色分析表皮改变、炎症浸润和纤维化情况，采用Fontana-Masson染色评估寄生虫。
在表皮真菌计数方面，Fontana-Masson染色优于苏木精-伊红染色（平均差异=0.14；p<0.05）。在真皮中观察到最明显的真菌病组织反应。治疗期间出现表皮变薄、肉芽肿浸润减少或消失、纤维化增加以及数量/形态学变化。
无法找到患者检查其当前皮肤状况。
本研究中证实的寄生虫和组织变化可反映伊曲康唑作用下的寄生虫-宿主动态关系。
