Suzuki H, Miyashita N, Moriwaki K, Kominami R, Muramatsu M, Kanehisa T, Bonhomme F, Petras M L, Yu Z C, Lu D Y
National Institute of Genetics, Japan.
Mol Biol Evol. 1986 Mar;3(2):126-37. doi: 10.1093/oxfordjournals.molbev.a040384.
Genetic variability of the nontranscribed spacer (NTS) region within ribosomal DNA repeating units in the various subspecies of Mus musculus was determined. Mice belonging to several laboratory mouse strains were examined by means of Southern blot hybridization with a mouse ribosomal DNA probe. This probe encompasses the 3' end of the 28S ribosomal RNA (rRNA) gene and the following spacer. Restriction enzyme digestions of the liver DNAs from various wild mice revealed that each of the subspecies has a unique pattern in the spacer encompassing a distance approximately 10 kb downstream from the ribosomal gene. These restriction patterns permit the classification of mouse subspecies and also provide insights into the origin of the laboratory mouse strains.
测定了小家鼠各亚种核糖体DNA重复单元内非转录间隔区（NTS）的遗传变异性。使用小鼠核糖体DNA探针通过Southern印迹杂交法对几种实验小鼠品系的小鼠进行了检测。该探针涵盖28S核糖体RNA（rRNA）基因的3'端及后续间隔区。对各种野生小鼠肝脏DNA进行的限制性酶切分析表明，每个亚种在核糖体基因下游约10 kb处的间隔区都有独特的模式。这些限制性模式有助于对小鼠亚种进行分类，也为实验小鼠品系的起源提供了见解。
