Gózd-Barszczewska Anna, Kozioł-Montewka Maria, Barszczewski Piotr, Młodzińska Agata, Humińska Kinga
Division of Cardiology with Cardiac Intensive Care Unit, Cardinal Stefan Wyszyński Hospital, Lublin, Poland.
Pope John Paul II State School of Higher Education, Biała Podlaska, Poland.
Ann Agric Environ Med. 2017 Sep 21;24(3):416-422. doi: 10.26444/aaem/75456. Epub 2017 Sep 8.
Cardiovascular diseases are the leading cause of death in Europe and worldwide. One of the most important risk factors for atherosclerosis are lipid metabolism disorders, in particular hipercholesterolaemia. The aim of the study was to determine the correlation between gut microbiota composition and atherosclerosis risk factors, so in order that it might be used as a biomarker for coronary artery disease diagnosis.
The study involved middle-aged men in eastern Poland with central obesity (n=20), subjects with atherosclerosis (n=15) and those with no cardiovascular diseases (n=5). The gut microbiota composition was determined using tag-encoded 16S rRNA gene using Illuminal MiSeq. Data were analyzed with the use of t-test.
Firmicutes (49.26%) and Bacteroidetes (44.46%) were the dominant Phyla in the middle-aged men in eastern Poland. Subjects with improper levels of total cholesterol were enriched in Prevotella (p=0.03) and decreased level of Clostridium (p=0.02). They also showed a falling tendency in Faecalibacterium (p=0.07). An upward trend was observed in Prevotella (p=0.07) in subjects with improper LDL-C values.
The study showed that intestinal microbiome is likely to play a role in the pathogenesis of atherosclerosis through its role in lipid metabolism. Bacterial genera of particular importance were Prevotella, Bacteroides, Clostridium, Faecalibacterium. However, further studies involving larger groups of subjects are required to confirm these observations.
心血管疾病是欧洲乃至全球的主要死因。动脉粥样硬化最重要的危险因素之一是脂质代谢紊乱，尤其是高胆固醇血症。本研究的目的是确定肠道微生物群组成与动脉粥样硬化危险因素之间的相关性，以便将其用作冠状动脉疾病诊断的生物标志物。
该研究纳入了波兰东部患有中心性肥胖的中年男性（n = 20）、患有动脉粥样硬化的受试者（n = 15）以及无心血管疾病的受试者（n = 5）。使用Illuminal MiSeq通过标签编码的16S rRNA基因确定肠道微生物群组成。数据采用t检验进行分析。
在波兰东部的中年男性中，厚壁菌门（49.26%）和拟杆菌门（44.46%）是主要的菌门。总胆固醇水平异常的受试者中普雷沃氏菌属增多（p = 0.03），梭菌属水平降低（p = 0.02）。他们的粪杆菌属也呈下降趋势（p = 0.07）。低密度脂蛋白胆固醇（LDL-C）值异常的受试者中普雷沃氏菌属呈上升趋势（p = 0.07）。
该研究表明肠道微生物群可能通过其在脂质代谢中的作用在动脉粥样硬化的发病机制中发挥作用。特别重要的细菌属有普雷沃氏菌属、拟杆菌属、梭菌属、粪杆菌属。然而，需要进一步纳入更大样本量受试者的研究来证实这些观察结果。
