Garner Allen L, Neculaes V Bogdan, Deminsky Maxim, Dylov Dmitry V, Joo Chulmin, Loghin Evelina R, Yazdanfar Siavash, Conway Kenneth R
School of Nuclear Engineering, Purdue University, West Lafayette, IN 47907, USA.
GE Global Research Center, 1 Research Circle, Niskayuna, NY 12309, USA.
Biochem Biophys Rep. 2015 Dec 2;5:168-174. doi: 10.1016/j.bbrep.2015.11.019. eCollection 2016 Mar.
Calculations indicate that selectively heating the extracellular media induces membrane temperature gradients that combine with electric fields and a temperature-induced reduction in the electropermeabilization threshold to potentially facilitate exogenous molecular delivery. Experiments by a wide-field, pulsed femtosecond laser with peak power density far below typical single cell optical delivery systems confirmed this hypothesis. Operating this laser in continuous wave mode at the same average power permeabilized many fewer cells, suggesting that bulk heating alone is insufficient and temperature gradients are crucial for permeabilization. This work suggests promising opportunities for a high throughput, low cost, contactless method for laser mediated exogenous molecule delivery without the complex optics of typical single cell optoinjection, for potential integration into microscope imaging and microfluidic systems.
计算表明，选择性加热细胞外介质会诱导膜温度梯度，该梯度与电场以及温度诱导的电通透阈值降低相结合，有可能促进外源性分子的递送。使用峰值功率密度远低于典型单细胞光递送系统的宽场脉冲飞秒激光进行的实验证实了这一假设。在相同平均功率下以连续波模式操作该激光时，通透的细胞数量要少得多，这表明仅体热是不够的，温度梯度对于通透至关重要。这项工作为一种高通量、低成本、非接触式的激光介导外源性分子递送方法提供了有前景的机会，该方法无需典型单细胞光注射的复杂光学元件，有望集成到显微镜成像和微流体系统中。
