Dhayakaran Rekha, Neethirajan Suresh, Weng Xuan
Bionano Laboratory, School of Engineering, University of Guelph, Guelph, Canada N1G 2W1.
Biochem Biophys Rep. 2016 Apr 7;6:149-157. doi: 10.1016/j.bbrep.2016.04.001. eCollection 2016 Jul.
Antimicrobial resistance is a great concern in the medical community, as well as food industry. Soy peptides were tested against bacterial biofilms for their antimicrobial activity. A high throughput drug screening assay was developed using microfluidic technology, RAMAN spectroscopy, and optical microscopy for rapid screening of antimicrobials and rapid identification of pathogens.
Synthesized PGTAVFK and IKAFKEATKVDKVVVLWTA soy peptides were tested against and using a microdilution assay. Microfluidic technology in combination with Surface Enhanced RAMAN Spectroscopy (SERS) and optical microscopy was used for rapid screening of soy peptides, pathogen identification, and to visualize the impact of selected peptides.
The PGTAVFK peptide did not significantly affect , although it had an inhibitory effect on L. above a concentration of 625 µM. IKAFKEATKVDKVVVLWTA was effective against both and L. above a concentration of 37.2 µM. High throughput drug screening assays were able to reduce the screening and bacterial detection time to 4 h. SERS spectra was used to distinguish the two bacterial species.
PGTAVFK and IKAFKEATKVDKVVVLWTA soy peptides showed antimicrobial activity against and L. . Development of high throughput assays could streamline the drug screening and bacterial detection process.
The results of this study show that the antimicrobial properties, biocompatibility, and biodegradability of soy peptides could possibly make them an alternative to the ineffective antimicrobials and antibiotics currently used in the food and medical fields. High throughput drug screening assays could help hasten pre-clinical trials in the medical field.
抗菌药物耐药性是医学界和食品行业极为关注的问题。对大豆肽针对细菌生物膜的抗菌活性进行了测试。利用微流控技术、拉曼光谱和光学显微镜开发了一种高通量药物筛选测定法，用于快速筛选抗菌剂和快速鉴定病原体。
使用微量稀释测定法，针对[具体细菌名称1]和[具体细菌名称2]测试合成的PGTAVFK和IKAFKEATKVDKVVVLWTA大豆肽。将微流控技术与表面增强拉曼光谱（SERS）和光学显微镜相结合，用于快速筛选大豆肽、鉴定病原体以及可视化所选肽的影响。
PGTAVFK肽对[具体细菌名称1]没有显著影响，尽管在浓度高于625μM时对[具体细菌名称2]有抑制作用。IKAFKEATKVDKVVVLWTA在浓度高于37.2μM时对[具体细菌名称1]和[具体细菌名称2]均有效。高通量药物筛选测定法能够将筛选和细菌检测时间缩短至4小时。利用SERS光谱区分这两种细菌。
PGTAVFK和IKAFKEATKVDKVVVLWTA大豆肽对[具体细菌名称1]和[具体细菌名称2]具有抗菌活性。高通量测定法的开发可以简化药物筛选和细菌检测过程。
本研究结果表明，大豆肽的抗菌特性、生物相容性和生物降解性可能使其成为食品和医疗领域目前使用的无效抗菌剂和抗生素的替代品。高通量药物筛选测定法有助于加快医学领域的临床前试验。
