鉴定在一种“野生型”菌株中导致负趋光性的突变。
Identification of the mutation responsible for negative phototaxis in a "wild-type" strain of .
作者信息
Ide Takahiro, Mochiji Shota, Ueki Noriko, Yamaguchi Katsushi, Shigenobu Shuji, Hirono Masafumi, Wakabayashi Ken-Ichi
机构信息
Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology, Yokohama 226-8503, Japan.
Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo 113-0033, Japan.
出版信息
Biochem Biophys Rep. 2016 Jul 20;7:379-385. doi: 10.1016/j.bbrep.2016.07.016. eCollection 2016 Sep.
The unicellular green alga is a model organism for various studies in biology. CC-124 is a laboratory strain widely used as a wild type. However, this strain is known to carry mutation, which causes cells to swim away from the light source (negative phototaxis), in contrast to the cells of other wild-type strains, which swim toward the light source (positive phototaxis). Here we identified the causative gene of () using AFLP-based gene mapping and whole genome next-generation sequencing. This gene encodes a 36-kDa protein containing a Fibronectin type III domain and a CHORD-Sgt1 (CS) domain. The gene product is localized to the cell body and not to flagella or basal body.
单细胞绿藻是生物学各种研究中的模式生物。CC-124是一种广泛用作野生型的实验室菌株。然而，已知该菌株携带突变，与其他向光源游动（正趋光性）的野生型菌株细胞相反，该突变导致细胞游离光源（负趋光性）。在这里，我们使用基于AFLP的基因定位和全基因组下一代测序鉴定了（）的致病基因。该基因编码一种含有纤连蛋白III型结构域和CHORD-Sgt1（CS）结构域的36 kDa蛋白质。该基因产物定位于细胞体，而非鞭毛或基体。
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