Ueki Noriko, Wakabayashi Ken-Ichi
Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology, Yokohama, Japan.
Bio Protoc. 2017 Jun 20;7(12):e2356. doi: 10.21769/BioProtoc.2356.
Phototaxis is a behavior in which organisms move toward or away from the light source (positive or negative phototaxis, respectively). It is crucial for phototrophic microorganisms to inhabit under proper light conditions for phototaxis. The unicellular green alga rapidly changes its swimming direction upon light illumination, and thus is a nice model organism for phototaxis research. Here we show two methods to assay phototaxis; one is a quick, easy and qualitative analysis, so-called the dish assay; and the other is a quantitative single-cell analysis.
趋光性是一种生物体朝着光源移动或背离光源移动的行为（分别为正趋光性或负趋光性）。对于光合微生物而言，在适宜的光照条件下进行趋光性活动至关重要。单细胞绿藻在光照时会迅速改变其游动方向，因此是用于趋光性研究的优良模式生物。在此，我们展示两种检测趋光性的方法；一种是快速、简便的定性分析方法，即所谓的平皿检测法；另一种是定量单细胞分析方法。
