Nagaki Yoshiaki, Ito Koichi, Kuwahara Masayoshi
Department of Veterinary Pathophysiology and Animal Health, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan.
Biochem Biophys Rep. 2016 Aug 31;8:157-161. doi: 10.1016/j.bbrep.2016.08.024. eCollection 2016 Dec.
Because we found that WTC rats might be resistant to streptozotocin (STZ), we have elucidated the mechanisms of resistant to the diabetogenic effects of STZ in the WTC rats. Dose response to STZ was evaluated with glucose levels. No significant changes in glucose level to STZ administration were observed in WTC rats. Insulin secretion by suppling glucose was preserved in WTC rats even after STZ administration. Although there was no significant difference in gene expression of both GLUT2 and Kir6.2, which were involved in STZ resistance, between WTC rats and Wistar rats, the expression of metallothionein 2a in pancreas and liver to STZ administration of WTC rats was significantly higher than that of Wistar rats. Moreover, alloxan did not induce diabetes in WTC rats as same as STZ. These results suggest that WTC rats might have powerful antioxidant property to protect β cells in pancreas. Because the STZ-resistant property is very close characteristics to human beings, WTC rats will become a useful animal model in diabetic researches.
因为我们发现WTC大鼠可能对链脲佐菌素（STZ）具有抗性，所以我们阐明了WTC大鼠对STZ致糖尿病作用产生抗性的机制。通过血糖水平评估对STZ的剂量反应。在WTC大鼠中，未观察到给予STZ后血糖水平有显著变化。即使在给予STZ后，WTC大鼠通过补充葡萄糖刺激的胰岛素分泌仍得以保留。虽然参与STZ抗性的葡萄糖转运蛋白2（GLUT2）和内向整流型钾离子通道蛋白6.2（Kir6.2）的基因表达在WTC大鼠和Wistar大鼠之间没有显著差异，但WTC大鼠胰腺和肝脏中金属硫蛋白2a对STZ给药后的表达显著高于Wistar大鼠。此外，与STZ一样，四氧嘧啶也不会在WTC大鼠中诱发糖尿病。这些结果表明，WTC大鼠可能具有强大的抗氧化特性来保护胰腺中的β细胞。由于STZ抗性特性与人类非常相似，WTC大鼠将成为糖尿病研究中一种有用的动物模型。
