Boonrungsiman Suwimon, Suchaoin Wongsakorn, Chetprayoon Paninee, Viriya-Empikul Nawin, Aueviriyavit Sasitorn, Maniratanachote Rawiwan
Nano Safety and Risk Assessment Laboratory, National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), 111 Thailand Science Park, Khlong Luang, Pathum Thani 12120, Thailand.
Nanomaterials for Energy and Catalysis Laboratory, National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), 111 Thailand Science Park, Khlong Luang, Pathum Thani 12120, Thailand.
Biochem Biophys Rep. 2017 Jan 5;9:203-210. doi: 10.1016/j.bbrep.2016.12.014. eCollection 2017 Mar.
We investigated cellular uptake behavior and biological responses of spherical and fibrous titanate nanomaterials in human monocyte THP-1 cells. Two titanate nanofibers (TiNFs), namely TF-1 and TF-2, were synthesized from anatase TiO nanoparticles (TNPs) via hydrothermal treatment. The synthesized TiNFs and TNPs were thoroughly characterized for their size, crystallinity, surface area and surface pH. TF-1 (∼2 µm in length) was amorphous with an acidic surface, while TF-2 (∼7 µm in length) was brookite with a basic surface. The results demonstrated that none of these titanate nanomaterials resulted in significant cytotoxicity, even at the highest doses tested (50 µg/ml), consistent with an absence of ROS generation and lack of change of mitochondrial membrane potential. While no cytotoxic effect was found in the titanate nanomaterials, TF-2 tended to decrease the proliferation of THP-1 cells. Furthermore, TF-2 resulted in an inflammatory cytokine response, as evidenced by dramatic induction of IL-8 and TNF-α release in TF2 but not TF-1 nor TNPs. These results suggest that shape of titanate nanomaterials plays an important role in cellular internalization, while surface pH may play a prominent role in inflammatory response in THP-1 cells.
我们研究了球形和纤维状钛酸盐纳米材料在人单核细胞THP-1细胞中的细胞摄取行为和生物学反应。通过水热处理，由锐钛矿型TiO纳米颗粒（TNPs）合成了两种钛酸盐纳米纤维（TiNFs），即TF-1和TF-2。对合成的TiNFs和TNPs的尺寸、结晶度、表面积和表面pH进行了全面表征。TF-1（长度约2 µm）为非晶态，表面呈酸性，而TF-2（长度约7 µm）为板钛矿型，表面呈碱性。结果表明，即使在测试的最高剂量（50 µg/ml）下，这些钛酸盐纳米材料均未导致明显的细胞毒性，这与未产生ROS以及线粒体膜电位未发生变化一致。虽然在钛酸盐纳米材料中未发现细胞毒性作用，但TF-2倾向于降低THP-1细胞的增殖。此外，TF-2引发了炎症细胞因子反应，TF-2中IL-8和TNF-α的释放显著增加，而TF-1和TNPs中则未出现这种情况。这些结果表明，钛酸盐纳米材料的形状在细胞内化过程中起重要作用，而表面pH可能在THP-1细胞的炎症反应中起显著作用。
