Department of Horticultural Science, University of Minnesota, 1970 Folwell Avenue, Saint Paul, MN, 55108, USA.
Microbial and Plant Genomics Institute, University of Minnesota, Saint Paul, MN, USA.
Planta. 2018 Jan;247(1):267-275. doi: 10.1007/s00425-017-2782-9. Epub 2017 Sep 27.
Leaf spray-MS minimizes tissue manipulation by effectively and quickly assessing in vivo specialized metabolites from intact plant tissue surfaces, including trichome metabolites. Intact leaves of Glycyrrhiza lepidota Pursh. (American licorice) were analyzed by direct electrospray leaf spray-MS, an ambient ionization technique. Comparison of metabolites detected by leaf spray-MS to those from LC-MS of bulk tissue and trichome enriched extracts showed dramatic differences. Leaf spray-MS results suggest that in specific situations this approach could complement traditional LC-MS analysis of bulk extracts. Leaf spray-MS as a metabolomics technique eliminates sample pretreatment and preparation allowing for rapid sampling in real time of living intact tissues. Specialized metabolites on the surface of tissues such as glandular trichomes metabolites are detected by leaf spray-MS.
叶喷雾质谱法通过有效、快速地评估完整植物组织表面（包括毛状体代谢物）的体内特殊代谢物，最大限度地减少了组织处理。采用直接电喷雾叶喷雾质谱法对甘草（美国甘草）的完整叶片进行了分析，这是一种环境离子化技术。通过叶喷雾质谱法检测到的代谢物与从大块组织和毛状体富集提取物的 LC-MS 检测到的代谢物进行比较，结果显示出明显的差异。叶喷雾质谱法的结果表明，在特定情况下，这种方法可以补充传统的大块提取物 LC-MS 分析。作为代谢组学技术的叶喷雾质谱法消除了样品预处理和准备，允许对活体完整组织进行实时快速采样。叶喷雾质谱法可以检测到组织表面的特殊代谢物，如腺毛代谢物。
