Olaya-Galán N N, Corredor-Figueroa A P, Guzmán-Garzón T C, Ríos-Hernandez K S, Salas-Cárdenas S P, Patarroyo M A, Gutierrez M F
PhD Programme in Biomedical and Biological Sciences,Universidad del Rosario,Bogotá,Colombia.
Grupo de Enfermedades Infecciosas, Laboratorio de Virología, Departamento de Microbiología,Pontificia Universidad Javeriana,Bogotá,Colombia.
Epidemiol Infect. 2017 Nov;145(15):3125-3130. doi: 10.1017/S0950268817002229. Epub 2017 Sep 28.
Bovine leukaemia virus (BLV) is the causative agent of enzootic bovine leucosis, which has been reported worldwide. BLV has been found recently in human tissue and it could have a significant impact on human health. A possible hypothesis regarding viral entry to humans is through the consumption of infected foodstuffs. This study was aimed at detecting the presence of BLV DNA in raw beef and fresh milk for human consumption. Nested PCR directed at the BLV gag gene (272 bp) was used as a diagnostic test. PCR products were confirmed by Sanger sequencing. Forty-nine per cent of the samples proved positive for the presence of proviral DNA. This is the first study highlighting the presence of the BLV gag gene in meat products for human consumption and confirms the presence of the viral DNA in raw milk, as in previous reports. The presence of viral DNA in food products could suggest that viral particles may also be found. Further studies are needed to confirm the presence of infected viral particles, even though the present findings could represent a first approach to BLV transmission to humans through foodstuff consumption.
牛白血病病毒（BLV）是地方流行性牛白血病的病原体，已在全球范围内报道。最近在人体组织中发现了BLV，它可能对人类健康产生重大影响。关于病毒进入人体的一种可能假设是通过食用受感染的食品。本研究旨在检测供人类食用的生牛肉和新鲜牛奶中BLV DNA的存在。针对BLV gag基因（272 bp）的巢式PCR用作诊断测试。PCR产物通过桑格测序进行确认。49%的样本被证明存在前病毒DNA。这是第一项强调供人类食用的肉类产品中存在BLV gag基因的研究，并证实了生牛奶中存在病毒DNA，正如之前的报道一样。食品中病毒DNA的存在可能表明也可能存在病毒颗粒。尽管目前的研究结果可能是BLV通过食用食品传播给人类的初步研究，但仍需要进一步研究来确认受感染病毒颗粒的存在。
