Rocheleau J P, Michel P, Lindsay L R, Drebot M, Dibernardo A, Ogden N H, Fortin A, Arsenault J
Groupe de recherche en épidémiologie des zoonoses et santé publique, Faculté de médecine vétérinaire,Université de Montréal,Saint-Hyacinthe, Québec,Canada.
National Microbiology Laboratory,Public Health Agency of Canada,Winnipeg, Manitoba,Canada.
Epidemiol Infect. 2017 Oct;145(14):2940-2948. doi: 10.1017/S0950268817002205. Epub 2017 Sep 28.
Periodic outbreaks of West Nile virus (WNV), Eastern equine encephalitis virus (EEEV) and to a lesser extent, California serogroup viruses (CSGV), have been reported in parts of Canada in the last decade. This study was designed to provide a broad assessment of arboviral activity in Quebec, Canada, by conducting serological surveys for these arboviruses in 196 horses, 1442 dogs and 485 humans. Sera were screened by a competitive enzyme linked immunosorbent assay and positive samples confirmed by plaque reduction neutralisation tests. The percentage of seropositive samples was 83·7%, 16·5%, 7·1% in horses, 18·8%, 0·6%, 0% in humans, 11·7%, 3·1%, 0% in adult dogs and 2·9%, 0·3%, 0% in juvenile dogs for CSGV, WNV and EEEV, respectively. Serological results in horses and dogs appeared to provide a meaningful assessment of risk to public health posed by multiple arboviruses.
在过去十年中，加拿大部分地区曾报告过西尼罗河病毒（WNV）、东部马脑炎病毒（EEEV）的周期性疫情，以及程度较轻的加利福尼亚血清群病毒（CSGV）疫情。本研究旨在通过对196匹马、1442只狗和485人进行这些虫媒病毒的血清学调查，对加拿大魁北克省的虫媒病毒活动进行全面评估。血清样本通过竞争性酶联免疫吸附试验进行筛查，阳性样本通过蚀斑减少中和试验进行确认。CSGV、WNV和EEEV的血清阳性样本百分比在马中分别为83.7%、16.5%、7.1%，在人类中分别为18.8%、0.6%、0%，在成年狗中分别为11.7%、3.1%、0%，在幼年狗中分别为2.9%、0.3%、0%。马和狗的血清学结果似乎为多种虫媒病毒对公共卫生造成的风险提供了有意义的评估。
