Hwang D H, Boudreau M, Chanmugam P
Human Nutrition Section, Louisiana State University Agricultural Center, Baton Rouge 70803.
J Nutr. 1988 Apr;118(4):427-37. doi: 10.1093/jn/118.4.427.
Rats were fed graded amounts of purified 18:3n-3 or fish oil concentrate in the presence of a constant amount of 18:2n-6 to evaluate the ability of 18:3n-3 compared with longer-chain n-3 fatty acids to inhibit 20:4n-6 metabolism in platelets and lungs. Dietary 18:3n-3 at a ratio of 0.28 (n-3 to n-6 fatty acids) suppressed levels of 20:4n-6 in lung and plasma phospholipids and the capacity of the tissues to synthesize cyclooxygenase-derived products in a dose-dependent fashion. At similar ratios of n-3 to n-6 dietary fatty acids, longer-chain n-3 fatty acids, which are abundant in fish oil, appear to be more effective than 18:3n-3 in suppressing 20:4n-6 levels and the capacity of the tissues to synthesize cyclooxygenase-derived products. Much greater amounts of 12-hydroxyeicosapentaenoic acid (12-HEPE) and 5-HEPE than of 12-hydroxyeicosatetraenoic acid (12-HETE) and 5-HETE appeared to be formed in tissues of the group receiving the highest amount of fish oil. These results suggest that ingestion of fish oil leads to increased formation of lipoxygenase-derived products of longer-chain n-3 fatty acids.
给大鼠喂食不同剂量的纯化18:3n-3或鱼油浓缩物，同时保持18:2n-6的量恒定，以评估18:3n-3与长链n-3脂肪酸相比抑制血小板和肺中20:4n-6代谢的能力。饮食中18:3n-3与n-6脂肪酸的比例为0.28时，可抑制肺和血浆磷脂中20:4n-6的水平以及组织合成环氧化酶衍生产物的能力，且呈剂量依赖性。在n-3与n-6饮食脂肪酸比例相似的情况下，鱼油中丰富的长链n-3脂肪酸在抑制20:4n-6水平和组织合成环氧化酶衍生产物的能力方面似乎比18:3n-3更有效。在接受最高剂量鱼油的组的组织中，生成的12-羟基二十碳五烯酸（12-HEPE）和5-HEPE的量比12-羟基二十碳四烯酸（12-HETE）和5-HETE多得多。这些结果表明，摄入鱼油会导致长链n-3脂肪酸的脂氧合酶衍生产物生成增加。
