Talahalli Ramaprasad R, Vallikannan Baskaran, Sambaiah Kari, Lokesh Belur R
Department of Lipid Science and Traditional Foods, Central Food Technological Research Institute, Mysore, 570 020, India.
Lipids. 2010 Sep;45(9):799-808. doi: 10.1007/s11745-010-3464-6. Epub 2010 Aug 24.
We made a comparative analysis of the uptake, tissue deposition and conversion of dietary alpha-linolenic acid (ALA) to its long chain metabolites eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) with preformed EPA + DHA. Diets containing linseed oil [with ALA at approximately 2.5 (4 g/kg diet), 5 (8 g/kg diet), 10 (16 g/kg diet), 25% (40 g/kg diet)] or fish oil [with EPA + DHA at approximately 1 (1.65 g/kg diet), 2.5 (4.12 g/kg diet), 5% (8.25 g/kg diet)] or groundnut oil without n-3 polyunsaturated fatty acids (n-3 PUFA) were fed to rats for 60 days. ALA and EPA + DHA in serum, liver, heart and brain increased with increments in the dietary ALA level. When preformed EPA + DHA were fed, the tissue EPA + DHA increased significantly compared to those given ALA. Normalized values from dietary n-3 PUFA to tissue EPA + DHA indicated that 100 mg of dietary ALA lead to accumulation of EPA + DHA at 2.04, 0.70, 1.91 and 1.64% of total fatty acids respectively in liver, heart, brain and serum. Similarly 100 mg of preformed dietary EPA + DHA resulted in 25.4, 23.8, 15.9 and 14.9% of total fatty acids in liver, heart, brain and serum respectively. To maintain a given level of EPA + DHA, the dietary ALA required is 12.5, 33.5, 8.3 and 9.1 times higher than the dietary EPA + DHA for liver, heart, brain and serum respectively. Hence the efficacy of precursor ALA is lower compared to preformed EPA + DHA in elevating serum and tissue long chain n-3 PUFA levels.
我们对膳食中的α-亚麻酸（ALA）及其长链代谢产物二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）与预先形成的EPA + DHA的摄取、组织沉积和转化进行了比较分析。将含有亚麻籽油[ALA含量约为2.5%（4 g/kg饲料）、5%（8 g/kg饲料）、10%（16 g/kg饲料）、25%（40 g/kg饲料）]或鱼油[EPA + DHA含量约为1%（1.65 g/kg饲料）、2.5%（4.12 g/kg饲料）、5%（8.25 g/kg饲料）]或不含n-3多不饱和脂肪酸（n-3 PUFA）的花生油的饲料喂养大鼠60天。血清、肝脏、心脏和大脑中的ALA以及EPA + DHA随着膳食ALA水平的增加而增加。当喂食预先形成的EPA + DHA时，与给予ALA的大鼠相比，组织中的EPA + DHA显著增加。从膳食n-3 PUFA到组织EPA + DHA的标准化值表明，100 mg膳食ALA分别导致肝脏、心脏、大脑和血清中EPA + DHA占总脂肪酸的比例为2.04%、0.70%、1.91%和1.64%。同样，100 mg预先形成的膳食EPA + DHA分别导致肝脏、心脏、大脑和血清中总脂肪酸的比例为25.4%、23.8%、15.9%和14.9%。为维持给定水平的EPA + DHA，肝脏、心脏、大脑和血清所需的膳食ALA分别比膳食EPA + DHA高12.5倍、33.5倍、8.3倍和9.1倍。因此，在提高血清和组织长链n-3 PUFA水平方面，前体ALA的功效低于预先形成的EPA + DHA。
