National Laboratory of Safety Evaluation (Environmental Assessment) of Veterinary Drugs, National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, College of Veterinary Medicine, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China.
National Laboratory of Safety Evaluation (Environmental Assessment) of Veterinary Drugs, National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, College of Veterinary Medicine, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China.
Ecotoxicol Environ Saf. 2018 Jan;147:817-823. doi: 10.1016/j.ecoenv.2017.09.055. Epub 2017 Sep 25.
The aim of this study was to investigate the fate of nine potential indicator antimicrobial resistance genes (ARGs) (sul1, sul2, tetB, tetM, ermB, ermF, fexA, cfr, intI1) and the diversity of bacterial communities in response to poultry manure applications to arable soil over a 90 day period. Quantitative real time PCR and Illumina high-throughput sequencing of 16S rDNA gene were used to quantify and trace ARG fate. The levels of all genes dramatically decreased over time and intI1, sul1, sul2 and tetM always had the greatest abundance and lowest dissipation rates. This indicated that more effort should be focused on the ARG elimination from manure rather than waiting for subsequent attenuation in the environment. Our sequencing results documented dramatic changes in the microbial community structure and diversity during these experiments. In poultry manure groups, Bacteroidetes and Actinobacteria were the two dominant phyla while Acidobacteria dominated the control groups. Moreover, the relative abundance of genera Corynebacterium, Pseudomonas, Ochrobactrum, Actinomadura and Bacillus, which contained potential opportunistic pathogens, changed over time suggesting that poultry manure not only strongly influenced bacterial community composition, but also selected specific bacterial communities. This study provides a glimpse of ARG fates and bacterial community diversity in soil after the application of poultry manure.
本研究旨在调查九种潜在的指示性抗生素耐药基因（ARGs）（sul1、sul2、tetB、tetM、ermB、ermF、fexA、cfr、intI1）的命运以及细菌群落对禽粪在 90 天内施用于耕地的响应变化。使用定量实时 PCR 和 Illumina 高通量测序 16S rDNA 基因来定量和追踪 ARG 命运。所有基因的水平随时间显著下降，而 intI1、sul1、sul2 和 tetM 始终具有最大的丰度和最低的消散率。这表明应该更加关注从粪便中消除 ARG，而不是等待随后在环境中衰减。我们的测序结果记录了在这些实验过程中微生物群落结构和多样性的巨大变化。在禽粪组中，拟杆菌门和放线菌门是两个主要的门，而酸杆菌门则在对照组中占主导地位。此外，包含潜在机会性病原体的棒状杆菌属、假单胞菌属、氧化单胞菌属、放线菌属和芽孢杆菌属的相对丰度随时间发生变化，表明禽粪不仅强烈影响细菌群落组成，而且还选择了特定的细菌群落。本研究提供了禽粪施用于土壤后 ARG 命运和细菌群落多样性的初步了解。
