高通量测序揭示了中国山东养殖废水中抗生素抗性细菌的丰度和多样性。
High throughput sequencing reveals the abundance and diversity of antibiotic-resistant bacteria in aquaculture wastewaters, Shandong, China.
作者信息
Zhong Chuanqing, Zhou Yingping, Zhao Jia, Fu Jiafang, Jiang Tianyi, Liu Bing, Chen Feiyong, Cao Guangxiang
机构信息
School of Municipal and Environmental Engineering, Shandong Jianzhu University, Jinan, China.
Resources and Environment Innovation Research Institute, Shandong Jianzhu University, Jinan, China.
出版信息
3 Biotech. 2021 Feb;11(2):104. doi: 10.1007/s13205-021-02656-4. Epub 2021 Jan 30.
UNLABELLED
An innovative investigation was undertaken into the abundance and diversity of high antibiotic-resistant bacteria in aquaculture waters in Shandong Province, China, through cumulation incubation, PCR amplification of 16S rDNA, and high-throughput sequencing. The results showed that and were abundant in the aquaculture waters, whereas other phylum including , , and had relatively lower abundance. Our studies revealed the presence of different bacteria in different locations in the aquaculture waters, most of which were resistant to multiple antibiotics. That is, the same microbial species from the same aquaculture wastewater can resist different antibiotics. Altogether, a considerable portion of the microbial community were found to be multi-drug resistant. It is essential that the spread of the antibiotic-resistant bacteria is controlled so that the distribution of antibiotic resistance genes to other environments is avoided.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s13205-021-02656-4.
未标注
通过累积培养、16S rDNA的PCR扩增和高通量测序，对中国山东省养殖水域中高抗生素抗性细菌的丰度和多样性进行了一项创新性调查。结果表明，[此处原文缺失具体菌名]和[此处原文缺失具体菌名]在养殖水域中含量丰富，而包括[此处原文缺失具体菌名]、[此处原文缺失具体菌名]、[此处原文缺失具体菌名]和[此处原文缺失具体菌名]在内的其他门的含量相对较低。我们的研究揭示了养殖水域不同位置存在不同的细菌，其中大多数对多种抗生素具有抗性。也就是说，来自同一养殖废水的相同微生物物种可以抵抗不同的抗生素。总的来说，发现相当一部分微生物群落具有多重耐药性。必须控制抗生素抗性细菌的传播，以避免抗生素抗性基因扩散到其他环境中。
补充信息
在线版本包含可在10.1007/s13205-021-02656-4获取的补充材料。
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