Zhang Shuang-Qing, Zhang Hai-Bo, Zhang Yan
Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, 11 Fucheng Road, Beijing 100048, China; National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, 27 Nanwei Road, Beijing 100050, China.
Hubei Provincial Key Laboratory of Yeast Function, 168 Chengdong Road, Yichang 443003, China.
Food Chem. 2018 Feb 15;241:1-6. doi: 10.1016/j.foodchem.2017.08.068. Epub 2017 Aug 22.
The in vivo determination of selenomethionine (SeMet) converted from selenium-enriched yeast (SeY) rather than the determination of in vitro hydrolyzed SeMet is a better parameter for the evaluation of SeY quality. A UPLC-MS/MS method was developed for the quantification of SeMet in rat plasma and the oral bioavailability of SeMet converted from SeY in rats. After a simple extraction with perchloric acid, SeMet and the internal standard methylselenocysteine (MSC) were separated on a C18 column with isocratic elution of water:acetonitrile:formic acid (99:1:0.1, v/v/v) and detected in the multiple reaction monitoring mode. The method was accurate (92.6-104.3%) and precise (1.8-11.0%), and the recovery was 79.4-95.4%. It was successfully applied to pharmacokinetic and bioavailability studies of SeY in rats following the intravenous administration of SeMet and intragastric administration of SeY. SeMet in vivo, converted from SeY, is reported for the first time, and the results suggested that the SeY bioavailability in rats is 87%.
对于富硒酵母（SeY）质量的评估，测定体内由富硒酵母转化而来的硒代蛋氨酸（SeMet），而非体外水解的硒代蛋氨酸，是一个更好的参数。建立了一种超高效液相色谱-串联质谱（UPLC-MS/MS）方法，用于定量大鼠血浆中的SeMet以及大鼠体内由SeY转化而来的SeMet的口服生物利用度。经高氯酸简单萃取后，SeMet和内标甲基硒代半胱氨酸（MSC）在C18柱上以水：乙腈：甲酸（99:1:0.1，v/v/v）等度洗脱进行分离，并在多反应监测模式下进行检测。该方法准确（92.6 - 104.3%）、精密（1.8 - 11.0%），回收率为79.4 - 95.4%。在静脉注射SeMet和灌胃给予SeY后，该方法成功应用于大鼠体内SeY的药代动力学和生物利用度研究。首次报道了体内由SeY转化而来的SeMet，结果表明大鼠体内SeY的生物利用度为87%。
