Department of Analytical Biochemistry, Institute of Food Technology and Nutrition, College of Natural Sciences, Rzeszow University, 35-601 Rzeszow, Poland.
Department of Bioenergetics and Food Analysis, Institute of Food Technology and Nutrition, College of Natural Sciences, Rzeszow University, 35-601 Rzeszow, Poland.
Molecules. 2020 Mar 24;25(6):1456. doi: 10.3390/molecules25061456.
The aim of this study was to characterize the interaction of chosen catechins ((+)-catechin, (-)-epigallocatechin (EGC), and (-)-epigallocatechin gallate (EGCG)) with human erythrocytes and their protective effects against oxidative damage of erythrocytes. Uptake of the catechins by erythrocytes was studied by fluorimetry, their interaction with erythrocyte membrane was probed by changes in erythrocyte osmotic fragility and in membrane fluidity evaluated with spin labels, while protection against oxidative damage was assessed by protection against hemolysis induced by permanganate and protection of erythrocyte membranes against lipid peroxidation and protein thiol group oxidation. Catechin uptake was similar for all the compounds studied. Accumulation of catechins in the erythrocyte membrane was demonstrated by the catechin-induced increase in osmotic resistance and rigidification of the erythrocyte membrane detected by spin labels 5-doxyl stearic acid and 16-doxyl stearic acid. (-)-Epigallocatechin and EGCG inhibited erythrocyte acetylcholinesterase (mixed-type inhibition). Catechins protected erythrocytes against permanganate-induced hemolysis, oxidation of erythrocyte protein thiol groups, as well as membrane lipid peroxidation. These results contribute to the knowledge of the beneficial effects of catechins present in plant-derived food and beverages.
本研究旨在描述几种儿茶素（（+）-儿茶素、（-）-表没食子儿茶素（EGC）和（-）-表没食子儿茶素没食子酸酯（EGCG））与人红细胞的相互作用，以及它们对红细胞氧化损伤的保护作用。通过荧光法研究儿茶素被红细胞摄取的情况，通过红细胞渗透脆性的变化和用自旋标记物评估的膜流动性变化来探测儿茶素与红细胞膜的相互作用，而通过保护红细胞免受高锰酸盐诱导的溶血、保护红细胞膜免受脂质过氧化和蛋白质巯基氧化来评估其对氧化损伤的保护作用。研究表明，所有被研究的化合物的儿茶素摄取情况相似。通过自旋标记物 5-二氧代硬脂酸和 16-二氧代硬脂酸检测到儿茶素诱导的红细胞膜渗透压升高和刚性增加，证明了儿茶素在红细胞膜中的积累。（-）-表没食子儿茶素和 EGCG 抑制红细胞乙酰胆碱酯酶（混合型抑制）。儿茶素可保护红细胞免受高锰酸盐诱导的溶血、红细胞蛋白巯基氧化以及膜脂质过氧化的影响。这些结果有助于了解植物源性食品和饮料中儿茶素的有益作用。
