芴甲氧羰基-甲硫氨酸及淀粉样纤维中蛋氨酸侧链的比较动力学
Comparative Dynamics of Methionine Side-Chain in FMOC-Methionine and in Amyloid Fibrils.
作者信息
Vugmeyster Liliya, Ostrovsky Dmitry
机构信息
Department of Chemistry, 1201 Larimer Street, University of Colorado at Denver, Denver, CO 80204, USA.
Department of Mathematics, 1201 Larimer Street, University of Colorado at Denver, Denver, CO 80204, USA.
出版信息
Chem Phys Lett. 2017 Apr;673:108-112. doi: 10.1016/j.cplett.2017.02.021. Epub 2017 Feb 14.
Abstract
We compared the dynamics of key methionine methyl groups in the water-accessible hydrophobic cavity of amyloid fibrils and Fluorenylmethyloxycarbonyl-Methionine (FMOC-Met), which renders general hydrophobicity to the environment without the complexity of the protein. Met35 in the hydrated cavity was recently found to undergo a dynamical cross-over from the dominance of methyl rotations at low temperatures to the dominance of diffusive motion of methyl axis at high temperatures. Current results indicate that in FMOC-Met this cross-over is suppressed, similar to what was observed for the dry fibrils, indicating that hydration of the cavity is driving the onset of the dynamical transition.
摘要
我们比较了淀粉样纤维的水可及疏水腔内关键甲硫氨酸甲基基团的动力学，以及芴甲氧羰基 - 甲硫氨酸（FMOC - Met）的动力学。FMOC - Met可赋予环境一般的疏水性，而不存在蛋白质的复杂性。最近发现，水合腔内的Met35经历了一个动力学转变，从低温下甲基旋转占主导转变为高温下甲基轴扩散运动占主导。目前的结果表明，在FMOC - Met中这种转变受到抑制，类似于在干燥纤维中观察到的情况，这表明腔的水合作用推动了动力学转变的开始。
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