Department of Bone and Joint Surgery, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, People's Republic of China.
IUBMB Life. 2017 Nov;69(11):867-876. doi: 10.1002/iub.1681. Epub 2017 Sep 29.
Long noncoding RNA (LncRNA) SOX2 overlapping transcript (SOX2-OT) has been shown to serve an oncogenic role in human lung cancer, hepatocellular carcinoma, and gastric cancer. However, the clinical significance and biological function of lncRNA SOX2-OT in osteosarcoma are still unclear. LncRNA SOX2-OT expression was measured in osteosarcoma tissues and cell lines. Loss-of-function and gain-of-function studies were performed to observe the effects of lncRNA SOX2-OT on osteosarcoma cells proliferation, migration, invasion, and expressions of cancer stem cell biomarker. The relationship between lncRNA SOX2-OT and SOX2 was analyzed in osteosarcoma tissues and cells. Rescued-function studies were conducted to confirm the role of SOX2 in the regulation of lncRNA SOX2-OT in osteosarcoma cells migration, invasion, and expression of cancer stem cell biomarkers. In our results, lncRNA SOX2-OT expression was increased in osteosarcoma tissues and cell lines, and associated with malignant status and overall survival in osteosarcoma patients. LncRNA SOX2-OT regulated osteosarcoma cells proliferation, migration, invasion, and expression of cancer stem cell biomarkers. LncRNA SOX2-OT positively regulated SOX2 expression in osteosarcoma cells and positively associated with SOX2 expression in osteosarcoma tissues. The rescued-function studies suggested that SOX2 is necessary for lncRNA SOX2-OT induced osteosarcoma cells migration, invasion, and expression of cancer stem cell biomarkers. In conclusion, lncRNA SOX2-OT is a prognostic biomarker for osteosarcoma patients and serves an oncogenic role to regulate osteosarcoma cells migration, invasion, and expression of cancer stem cell biomarkers. © 2017 IUBMB Life, 69(11):867-876, 2017.
长链非编码 RNA (LncRNA) SOX2 重叠转录物 (SOX2-OT) 已被证明在人类肺癌、肝细胞癌和胃癌中发挥致癌作用。然而，lncRNA SOX2-OT 在骨肉瘤中的临床意义和生物学功能仍不清楚。测量骨肉瘤组织和细胞系中的 lncRNA SOX2-OT 表达。进行功能丧失和功能获得研究，观察 lncRNA SOX2-OT 对骨肉瘤细胞增殖、迁移、侵袭和癌症干细胞标志物表达的影响。分析骨肉瘤组织和细胞中 lncRNA SOX2-OT 与 SOX2 的关系。进行挽救功能研究，以确认 SOX2 在调节骨肉瘤细胞迁移、侵袭和表达癌症干细胞标志物中的作用。在我们的研究结果中，lncRNA SOX2-OT 在骨肉瘤组织和细胞系中表达增加，与骨肉瘤患者的恶性状态和总生存相关。lncRNA SOX2-OT 调节骨肉瘤细胞增殖、迁移、侵袭和表达癌症干细胞标志物。lncRNA SOX2-OT 正向调节骨肉瘤细胞中的 SOX2 表达，并与骨肉瘤组织中的 SOX2 表达呈正相关。挽救功能研究表明，SOX2 是 lncRNA SOX2-OT 诱导的骨肉瘤细胞迁移、侵袭和表达癌症干细胞标志物所必需的。总之，lncRNA SOX2-OT 是骨肉瘤患者的预后生物标志物，发挥致癌作用，调节骨肉瘤细胞迁移、侵袭和表达癌症干细胞标志物。
