Bethlenfalvay N C, Lima J E, Waldrup T
J Cell Physiol. 1984 Jul;120(1):69-74. doi: 10.1002/jcp.1041200110.
Opossum erythrocytes filtered through cellulose columns were used to estimate their permeability to D-glucose and optimum inorganic phosphate requirement for D-glucose utilization at pH 7.4 and 8.1. D-Glucose readily penetrated opossum red cells; there was no measurable difference whether plasma or electrolyte solution served as the suspending medium. Optimum extracellular inorganic phosphate concentration for glucose utilization as indicated by red cell lactate production was pH-dependent, with a sharp optimum of 30 mmol/liter at pH 8.1. Whereas glucose, fructose, mannose, dihydroxyacetone, adenosine, and inosine were readily utilized at pH 7.4 and Pi 30 mmol/liter as shown by net lactate and ATP production by the red cells, galactose and ribose as substrates were not metabolized. In electrolyte, Pi 30 mmol/liter, and pH 7.4 glucose utilization by opossum red cells averaged 3.5 mumol, at pH 8.1, 9.5 mumol/ml cells/hr were utilized. Red cells suspended in leukocyte-free plasma utilized D-glucose at a rate of 3.0 mumol/ml/hr at pH 7.5. Seven percent of D-glucose flowed through the pentose phosphate pathway; this rate increased 11-fold by methylene blue stimulation. The amount of D-glucose recycled through the pentose phosphate pathway increased 300-fold in the presence of the redox dye.
通过纤维素柱过滤的负鼠红细胞被用于评估其对D-葡萄糖的通透性以及在pH 7.4和8.1条件下利用D-葡萄糖所需的最佳无机磷酸盐浓度。D-葡萄糖很容易穿透负鼠红细胞；无论是血浆还是电解质溶液作为悬浮介质，都没有可测量的差异。红细胞乳酸生成所表明的葡萄糖利用的最佳细胞外无机磷酸盐浓度依赖于pH值，在pH 8.1时，最佳浓度为30 mmol/升。红细胞产生的净乳酸和ATP表明，在pH 7.4和无机磷酸盐30 mmol/升的条件下，葡萄糖、果糖、甘露糖、二羟基丙酮、腺苷和肌苷很容易被利用，而半乳糖和核糖作为底物则不被代谢。在电解质、无机磷酸盐30 mmol/升和pH 7.4的条件下，负鼠红细胞对葡萄糖的利用率平均为3.5 μmol，在pH 8.1时，每毫升细胞每小时的利用率为9.5 μmol。悬浮在无白细胞血浆中的红细胞在pH 7.5时以3.0 μmol/毫升/小时的速率利用D-葡萄糖。7%的D-葡萄糖通过磷酸戊糖途径流动；通过亚甲蓝刺激，该速率增加了11倍。在氧化还原染料存在的情况下，通过磷酸戊糖途径循环的D-葡萄糖量增加了300倍。
