Gu Peng-Ying, Yu Feng, Jin Song, Yang Qian, Su Jing, Chen Yan, Zhao Liang, Hu Shi-Lian
Department of Geriatric Medicine, Anhui Provincial Hospital, Anhui Medical University, Hefei, Anhui 230001, P.R. China.
Department of Neurology, Anhui Provincial Hospital, Anhui Medical University, Hefei, Anhui 230001, P.R. China.
Exp Ther Med. 2017 Sep;14(3):2603-2607. doi: 10.3892/etm.2017.4838. Epub 2017 Jul 25.
The aim of the study was to investigate the correlation between the serum undercarboxylated osteocalcin (ucOC) level and the blood biochemistry and cognitive impairment in rats with type 2 diabetes mellitus (T2DM). Sprague-Dawley (SD) rats were randomly divided into the normal control group (NC) and type 2 DM group. DM group received the high-fat and high-sugar diet combined with the intraperitoneal injection of low-dose STZ to establish the type 2 DM rat model. After 12 weeks of feeding, a Morris water maze was used to observe the rats' cognitive ability, and the levels of blood lipid, ucOC, insulin and adiponectin in the two groups were measured. The results showed that blood glucose of rats in DM group was increased significantly at 2-12 weeks (p<0.01) and the body weight was significantly increased at 4-12 weeks (p<0.01). The levels of serum triglyceride (TG), total cholesterol, low-density lipoprotein and insulin in rats in DM group were significantly increased compared with those in NC group (p<0.01) and the levels of high-density lipoprotein, adiponectin and ucOC were significantly decreased compared with those in the NC group (p<0.01). The place navigation and spatial exploration capacities of rats in DM group were significantly decreased compared with those in NC group (p<0.01). In the DM group, the place navigation and spatial exploration capacities of rats in the low ucOC group were significantly decreased compared with those in the high ucOC group (p<0.01). Additionally, single-factor correlation analysis revealed that ucOC was negatively correlated with blood glucose, TG and escape latency (p<0.01), but was positively correlated with adiponectin, residence time in target quadrant and traversing times (p<0.05 or p<0.01). In conclusion, the decreased serum ucOC level in rats with type 2 diabetes mellitus has a certain correlation with cognitive impairment.
本研究旨在探讨2型糖尿病（T2DM）大鼠血清羧化不全骨钙素（ucOC）水平与血液生化指标及认知功能障碍之间的相关性。将Sprague-Dawley（SD）大鼠随机分为正常对照组（NC）和2型糖尿病组。糖尿病组接受高脂高糖饮食并腹腔注射低剂量链脲佐菌素以建立2型糖尿病大鼠模型。喂养12周后，采用Morris水迷宫观察大鼠的认知能力，并检测两组大鼠的血脂、ucOC、胰岛素和脂联素水平。结果显示，糖尿病组大鼠在2 - 12周时血糖显著升高（p<0.01），在4 - 12周时体重显著增加（p<0.01）。与NC组相比，糖尿病组大鼠血清甘油三酯（TG）、总胆固醇、低密度脂蛋白和胰岛素水平显著升高（p<0.01），高密度脂蛋白、脂联素和ucOC水平显著降低（p<0.01）。与NC组相比，糖尿病组大鼠的位置导航和空间探索能力显著降低（p<0.01）。在糖尿病组中，低ucOC组大鼠的位置导航和空间探索能力与高ucOC组相比显著降低（p<0.01）。此外，单因素相关性分析显示，ucOC与血糖、TG和逃避潜伏期呈负相关（p<0.01），但与脂联素、在目标象限的停留时间和穿越次数呈正相关（p<0.05或p<0.01）。综上所述，2型糖尿病大鼠血清ucOC水平降低与认知功能障碍具有一定的相关性。
