Li Zhiming, Wang Yumei
Center for Information Technology, Hexi University, 846 Huancheng North Road, Zhangye 734000, PR China.
Department of Pharmacology, Medical College, Hexi University, 24 Danxia East Road, Zhangye 734000, PR China.
Toxicol Rep. 2015 Aug 4;2:1111-1116. doi: 10.1016/j.toxrep.2015.07.019. eCollection 2015.
This study was designed to evaluate whether NADPH oxidase inhibitor (apocynin) preconditioning induces expression of Src homology-2 domain-containing phosphatase-1 (SHP-1) to protect against renal ischemia/reperfusion (I/R) injury (RI/RI) in rats. Rats were pretreated with 50 mg/kg apocynin, then subjected to 45 min ischemia and 24 h reperfusion. The results indicated that apocynin preconditioning improved the recovery of renal function and nitroso-redox balance, reduced oxidative stress injury and inflammation damage, and upregulated expression of SHP-1 as compared to RI/RI group. Therefore our study demonstrated that apocynin preconditioning provided a protection to the kidney against I/R injury in rats partially through inducing expression of SHP-1.
本研究旨在评估烟酰胺腺嘌呤二核苷酸磷酸（NADPH）氧化酶抑制剂（白杨素）预处理是否通过诱导含Src同源2结构域的磷酸酶-1（SHP-1）表达来保护大鼠肾脏免受缺血/再灌注（I/R）损伤（RI/RI）。大鼠用50mg/kg白杨素预处理，然后进行45分钟缺血和24小时再灌注。结果表明，与RI/RI组相比，白杨素预处理改善了肾功能恢复和亚硝基氧化还原平衡，减少了氧化应激损伤和炎症损伤，并上调了SHP-1的表达。因此，我们的研究表明，白杨素预处理部分通过诱导SHP-1表达为大鼠肾脏提供了对I/R损伤的保护作用。
