Jacobson S H, Källenius G, Lins L E, Svenson S B
Department of Medicine, Karolinska Hospital, Stockholm, Sweden.
J Urol. 1988 May;139(5):900-3. doi: 10.1016/s0022-5347(17)42710-8.
The adherence of fluorescein isothiocyanate-labeled P-fimbriated Escherichia coli to uroepithelial cells from 19 women with chronic pyelonephritis was determined with the fluorescence-activated cell sorting technique. The application of this method has made it possible to study bacterial binding to a large number of cells. Renal function was determined in all patients and the recurrences of P-fimbriated Escherichia coli bacteriuria, cystitis and acute pyelonephritis during a 3-year followup were studied. We found a significant correlation between the P-fimbriae receptor accessibility on uroepithelial cells and glomerular filtration rate (r equals -0.75, p less than 0.001). Uroepithelial cells from the patients with chronic pyelonephritis and renal insufficiency had a higher binding capacity of P-fimbriated Escherichia coli than uroepithelial cells from patients with a normal glomerular filtration rate. There was no correlation between kidney function and the availability of P-fimbriae receptors in a control group of patients with polycystic kidney disease.
采用荧光激活细胞分选技术，测定了19例慢性肾盂肾炎女性患者尿路上皮细胞对异硫氰酸荧光素标记的P菌毛大肠杆菌的黏附情况。该方法的应用使得研究细菌与大量细胞的结合成为可能。对所有患者进行了肾功能测定，并研究了3年随访期间P菌毛大肠杆菌菌尿、膀胱炎和急性肾盂肾炎的复发情况。我们发现，尿路上皮细胞上P菌毛受体的可及性与肾小球滤过率之间存在显著相关性（r = -0.75，p < 0.001）。慢性肾盂肾炎和肾功能不全患者的尿路上皮细胞对P菌毛大肠杆菌的结合能力高于肾小球滤过率正常患者的尿路上皮细胞。多囊肾病患者对照组的肾功能与P菌毛受体的可及性之间无相关性。
