用于 cryo-EM 淘金热的工具：从 cryo-EM 映射到原子模型。

Theoretical Biology and Biophysics Group, Los Alamos National Laboratory, Los Alamos, U.S.A.

New Mexico Consortium, Los Alamos, U.S.A.

Biosci Rep. 2017 Dec 5;37(6). doi: 10.1042/BSR20170072. Print 2017 Dec 22.

As cryo-electron microscopy (cryo-EM) enters mainstream structural biology, the demand for fitting methods is high. Here, we review existing flexible fitting methods for cryo-EM. We discuss their importance, potential concerns and assessment strategies. We aim to give readers concrete descriptions of cryo-EM flexible fitting methods with corresponding examples.

随着冷冻电子显微镜（cryo-EM）进入主流结构生物学领域，对拟合方法的需求很高。本文综述了现有的 cryo-EM 柔性拟合方法，讨论了它们的重要性、潜在问题和评估策略。旨在为读者提供具体的 cryo-EM 柔性拟合方法描述，并给出相应的实例。