MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge CB2 0QH, England.
Acta Crystallogr D Struct Biol. 2017 Jun 1;73(Pt 6):496-502. doi: 10.1107/S2059798316019276. Epub 2017 Apr 20.
The formal concept of a workflow to single-particle analysis of cryo-electron microscopy (cryo-EM) images in the RELION program is described. In this approach, the structure-determination process is considered as a graph, where intermediate results in the form of images or metadata are the vertices, and different functionalities of the program are the edges. The new implementation automatically logs all user actions, facilitates file management and disk cleaning, and allows convenient browsing of the history of a project. Moreover, new functionality to iteratively execute consecutive jobs allows on-the-fly image processing, which will lead to more efficient data acquisition by providing faster feedback on data quality. The possibility of exchanging data-processing procedures among users will contribute to the development of standardized image-processing procedures, and hence increase accessibility for new users in this rapidly expanding field.
描述了在 RELION 程序中对冷冻电子显微镜 (cryo-EM) 图像进行单颗粒分析的工作流的正式概念。在这种方法中，结构确定过程被视为一个图，其中以图像或元数据形式呈现的中间结果为顶点，而程序的不同功能为边。新实现自动记录所有用户操作，方便文件管理和磁盘清理，并允许方便地浏览项目历史记录。此外，新的迭代执行连续作业的功能允许实时图像处理，这将通过提供对数据质量的更快反馈来更有效地采集数据。在用户之间交换数据处理过程的可能性将有助于标准化图像处理过程的发展，从而增加该快速扩展领域对新用户的可及性。
