Rettig W J, Garin-Chesa P, Beresford H R, Oettgen H F, Melamed M R, Old L J
Laboratory of Human Cancer Immunology, Memorial Sloan-Kettering Cancer Center, New York, NY 10021.
Proc Natl Acad Sci U S A. 1988 May;85(9):3110-4. doi: 10.1073/pnas.85.9.3110.
Normal differentiation and malignant transformation of human cells are characterized by specific changes in surface antigen phenotype. In the present study, we have defined six cell-surface antigens of human sarcomas and normal mesenchymal cells, by using mixed hemadsorption assays and immunochemical methods for the analysis of cultured cells and immunohistochemical staining for the analysis of normal tissues and greater than 200 tumor specimens. Differential patterns of F19 (Mr, 120,000/95,000 glycoprotein), F24 (Mr, 95,000 glycoprotein), G171 (Mr, 75,000 glycoprotein), G253 (Mr, 90,000 glycoprotein), S5 (Mr, 120,000 glycoprotein), and Thy-1 (Mr, 25,000 glycoprotein) antigen expression were found to characterize (i) subsets of cultured sarcoma cell lines, (ii) cultured fibroblasts derived from various organs, (iii) normal resting and activated mesenchymal tissues, and (iv) sarcoma and nonmesenchymal tumor tissues. These results provide a basic surface antigenic map for cultured mesenchymal cells and mesenchymal tissues and permit the classification of human sarcomas according to their antigenic phenotypes.
人类细胞的正常分化和恶性转化具有表面抗原表型的特定变化特征。在本研究中，我们通过混合血细胞吸附试验和免疫化学方法分析培养细胞，以及免疫组织化学染色分析正常组织和200多个肿瘤标本，确定了人类肉瘤和正常间充质细胞的六种细胞表面抗原。发现F19（分子量120,000/95,000糖蛋白）、F24（分子量95,000糖蛋白）、G171（分子量75,000糖蛋白）、G253（分子量90,000糖蛋白）、S5（分子量120,000糖蛋白）和Thy-1（分子量25,000糖蛋白）抗原表达的差异模式可表征：（i）培养的肉瘤细胞系亚群；（ii）来自不同器官的培养成纤维细胞；（iii）正常静息和活化的间充质组织；以及（iv）肉瘤和非间充质肿瘤组织。这些结果为培养的间充质细胞和间充质组织提供了基本的表面抗原图谱，并允许根据其抗原表型对人类肉瘤进行分类。
