反刍动物中生长抑素的免疫化学特性分析

Immunochemical characterisation of somatostatin in ruminants.

作者信息

Darvodelsky A M, Davey M W, Reid A M, Titchen D A, Wang X

机构信息

Department of Veterinary Physiology, University of Sydney, N.S.W., Australia.

出版信息

Regul Pept. 1988 Feb;20(2):161-70. doi: 10.1016/0167-0115(88)90049-3.

DOI:10.1016/0167-0115(88)90049-3

PMID:2896372

Abstract

Following development and validation of a radioimmunoassay for somatostatin, the immunoreactivity of this peptide in the plasma of ruminants was measured and the levels in sheep were 9-31 pM (mean 18 +/- 7 pM, n = 48), in lambs 10-54 pM (mean 25 +/- 10 pM, n = 18) and in calves 5-35 pM (mean 12 +/- 6 pM, n = 22). Somatostatin-like immunoreactivity was present in sheep in high concentrations in the antrum (2342 +/- 280 pmol/g wet weight), duodenum (446 +/- 73 pmol/g) and pancreas (832 +/- 208 pmol/g). Lower concentrations (6-150 pmol/g) were found in other regions of the gastrointestinal tract. Molecular sieve chromatography on Bio-Gel P-10 showed that while most of the somatostatin in the antrum was somatostatin-14, in the duodenum about 30% of the total immunoreactivity was somatostatin-28.

摘要

在生长抑素放射免疫分析方法建立并验证之后，测定了反刍动物血浆中该肽的免疫反应性，绵羊血浆中的水平为9 - 31皮摩尔（平均18 ± 7皮摩尔，n = 48），羔羊为10 - 54皮摩尔（平均25 ± 10皮摩尔，n = 18），犊牛为5 - 35皮摩尔（平均12 ± 6皮摩尔，n = 22）。生长抑素样免疫反应性在绵羊的胃窦（2342 ± 280皮摩尔/克湿重）、十二指肠（446 ± 73皮摩尔/克）和胰腺（832 ± 208皮摩尔/克）中浓度较高。在胃肠道的其他区域发现浓度较低（6 - 150皮摩尔/克）。在Bio - Gel P - 10上进行的分子筛层析显示，虽然胃窦中的大部分生长抑素是生长抑素 - 14，但十二指肠中总免疫反应性的约30%是生长抑素 - 28。