Laboratório de Processos Biológicos (LPB), Escola de Engenharia de São Carlos (EESC), Universidade de São Paulo (USP), Av. João Dagnone, 1100, Santa Angelina, São Carlos, São Paulo 13563-120, Brazil.
Laboratório Nacional de Ciência e Tecnologia do Bioetanol (CTBE), Centro Nacional de Pesquisa em Energia e Materiais (CNPEM), Rua Giuseppe Máximo Scolfaro, 10000, Polo II de Alta Tecnologia, P.O. Box 6170, Campinas, São Paulo 13083-970, Brazil.
Bioresour Technol. 2018 Jan;247:426-433. doi: 10.1016/j.biortech.2017.09.121. Epub 2017 Sep 21.
This study aimed to better understand the role of different microbial groups and the determining fermentation pathways in a biohydrogen (bioH)-producing reactor fed with sugarcane vinasse by using next-generation sequencing and principal component analysis (PCA). Both microbial structure and dynamics were characterized. The highest bioH production levels were associated with the Thermoanaerobacterium genus, whilst lactate-producing bacteria comprised the dominant genera (e.g. Lactobacillus and Leuconostoc) within the reactor at both stable and unstable bioH-producing periods. PCA further revealed that the fermentation of lactate played a dual role in the reactor, as both bioH-producing (acetate+lactate→butyrate+bioH) and non-bioH-producing (lactate→propionate+acetate) routes could be observed. Overall, the results suggested that lactate is the primary alternative carbon source in vinasse-fed systems subjected to carbohydrate-shortage conditions.
本研究旨在通过使用下一代测序和主成分分析（PCA），更好地了解在以甘蔗废糖蜜为原料的产氢（bioH）反应器中，不同微生物群和决定发酵途径的作用。对微生物结构和动态进行了表征。最高的 bioH 生产水平与热厌氧菌属有关，而在稳定和不稳定的 bioH 生产期间，产乳酸细菌构成了反应器中的主要属（如乳酸杆菌属和肠球菌属）。PCA 进一步表明，乳酸的发酵在反应器中起双重作用，因为可以观察到产 bioH 的途径（乙酸盐+乳酸→丁酸盐+bioH）和非产 bioH 的途径（乳酸→丙酸+乙酸盐）。总的来说，结果表明，在碳水化合物短缺条件下以废糖蜜为原料的系统中，乳酸是主要的替代碳源。
