de Souza Moraes Bruna, Mary Dos Santos Graciete, Palladino Delforno Tiago, Tadeu Fuess Lucas, José da Silva Ariovaldo
Interdisciplinary Center of Energy Planning (NIPE), University of Campinas (Unicamp), Rua Cora Coralina, 330, Cidade Universitária, Campinas, São Paulo 13083-896, Brazil.
School of Agricultural Engineering (FEAGRI), University of Campinas (Unicamp), Av. Cândido Rondon, 501, Barão Geraldo, Campinas, São Paulo 13083-875, Brazil.
J Biosci Bioeng. 2019 May;127(5):594-601. doi: 10.1016/j.jbiosc.2018.10.008. Epub 2018 Nov 9.
The role of sugarcane vinasse as a nutrient source and the impacts of different inoculum pretreatment methods (acid-thermal and thermal treatment) were assessed in acidogenic systems aiming to produce value-added short-chain organic acids (SCOA) and alcohols. In-depth microbiome characterization was also conducted by high-throughput sequencing of the 16S rRNA gene using the Miseq Illumina platform. SCOA production was 47.3 % higher in vinasse-fed reactors, with isobutyric (up to 10.3 g L) and butyric (up to 10.6 g L) acids as the primary metabolites most likely resulting from lactate conversion. Ethanol comprised the main product from solventogenic pathways in all conditions, with values ranging between 2.7 and 5.2 g L, whereas no butanol was detected. Microbial analyses revealed high relative abundance values for the Clostridium, Lactobacillus, Bacillus and Ruminococcus genera, with the predominance of the Clostridium genus (17%) in acid-thermal treatment reactors and the Lactobacillus genus (37%) in thermal treatment reactors. Overall, vinasse proved to be a suitable substrate for value-added SCOA production, which characterizes a potential management approach to this wastewater stream. In this sense, the biochemical production of butyrate from vinasse could diversify the product portfolio of sugarcane biorefineries, also minimizing bioenergy losses by converting residual carbon fractions.
在旨在生产增值短链有机酸（SCOA）和醇类的产酸系统中，评估了甘蔗酒糟作为营养源的作用以及不同接种物预处理方法（酸热处理和热处理）的影响。还使用Illumina Miseq平台通过16S rRNA基因的高通量测序对微生物群落进行了深入表征。在以酒糟为进料的反应器中，SCOA产量高出47.3%，异丁酸（高达10.3 g/L）和丁酸（高达10.6 g/L）是最有可能由乳酸转化产生的主要代谢产物。在所有条件下，乙醇都是溶剂生成途径的主要产物，产量在2.7至5.2 g/L之间，未检测到丁醇。微生物分析显示，梭菌属、乳杆菌属、芽孢杆菌属和瘤胃球菌属的相对丰度值较高，在酸热处理反应器中梭菌属占主导（17%），在热处理反应器中乳杆菌属占主导（37%）。总体而言，酒糟被证明是生产增值SCOA的合适底物，这为该废水流提供了一种潜在的管理方法。从这个意义上说，从酒糟中生化生产丁酸盐可以使甘蔗生物精炼厂的产品组合多样化，还能通过转化残余碳组分将生物能源损失降至最低。
