Department of Medical Microbiology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.
Department of Pathology, University of Debrecen, Debrecen, Hungary.
Pathobiology. 2018;85(3):179-185. doi: 10.1159/000481174. Epub 2017 Sep 29.
BACKGROUND/AIMS: The pathogenesis of the human polyomavirus (PyV) KI, WU, MW, and STL has not been elucidated yet. Respiratory transmission is suggested, but the site of the replication, tissue, and cell tropism is not clarified. KIPyV and WUPyV DNA and/or antigen were detected in normal lung tissues previously by others. In fact, a KIPyV DNA sequence was found in lung cancer samples. Up to date, there is no publication about the DNA prevalence of MWPyV and STLPyV neither in normal nor in cancerous lung tissues. The aim of the present study was to examine the DNA prevalence of these polyomaviruses in cancerous and non-cancerous lung tissue samples, in order to study the possible site for viral replication and/or persistence, and the potential association of these viruses with lung carcinogenesis as well.
100 cancerous and 47 non-cancerous, formalin-fixed paraffin-embedded lung tissue samples were studied for KIPyV, WUPyV, MWPyV, and STLPyV by real-time PCR.
Neither of the viruses was found in samples from small-cell, non-small-cell (adenocarcinoma, squamous-cell carcinoma and large-cell neuroendocrine lung cancer), mixed-type and non-differentiated lung carcinoma, and non-cancerous lung tissues (from patients with pneumonia, emphysema and fibrosis).
背景/目的：人类多瘤病毒（PyV）KI、WU、MW 和 STL 的发病机制尚未阐明。有研究表明呼吸道传播可能与该病毒有关，但病毒的复制部位、组织和细胞嗜性尚不清楚。此前已有研究人员在正常肺组织中检测到 KIPyV 和 WUPyV 的 DNA 和/或抗原。事实上，在肺癌样本中发现了 KIPyV 的 DNA 序列。迄今为止，尚无关于 MWPyV 和 STLPyV 在正常和癌性肺组织中 DNA 流行率的报道。本研究旨在检测这些多瘤病毒在癌性和非癌性肺组织样本中的 DNA 流行率，以研究病毒复制和/或持续存在的可能部位，以及这些病毒与肺癌发生的潜在关联。
采用实时 PCR 法对 100 例癌性和 47 例非癌性福尔马林固定石蜡包埋肺组织样本进行 KIPyV、WUPyV、MWPyV 和 STLPyV 检测。
在小细胞癌、非小细胞癌（腺癌、鳞状细胞癌和大细胞神经内分泌肺癌）、混合型和未分化型肺癌以及非癌性肺组织（肺炎、肺气肿和纤维化患者）中均未发现这些病毒。
