Bounouala Fatima Zohra, Roudj Salima, Karam Nour-Eddine, Recio Isidra, Miralles Beatriz
Instituto de Investigación en Ciencias de la Alimentación, Consejo Superior de Investigaciones Científicas - Universidad Autónoma de Madrid (CIAL, CSIC-UAM) , Nicolás Cabrera 9, 28049 Madrid, Spain.
Laboratoire de Biologie des Microorganismes et Biotechnologie (LBMB). Université d'Oran 1 Ahmed Ben Bella , BP 1524 Oran El Mnaouer, 31000, Oran, Algérie.
J Agric Food Chem. 2017 Oct 25;65(42):9324-9332. doi: 10.1021/acs.jafc.7b03203. Epub 2017 Oct 12.
Casein from ovine and bovine milk were hydrolyzed with two extracellular protease preparations from Lactobacillus brevis and Lactococcus lactis. The hydrolysates were analyzed by HPLC-MS/MS for peptide identification. A strain-dependent peptide profile could be observed, regardless of the casein origin, and the specificity of these two proteases could be computationally ascribed. The cleavage pattern yielding phenylalanine, leucine, or tyrosine at C-terminal appeared both at L. lactis and Lb. brevis hydrolysates. However, the cleavage C-terminal to lysine was favored with Lb. brevis protease. The hydrolysates showed ACE-inhibitory activity with IC in the 16-70 μg/mL range. Ovine casein hydrolysates yielded greater ACE-inhibitory activity. Previously described antihypertensive and opioid peptides were found in these ovine and bovine casein hydrolysates and prediction of the antihypertensive activity of the sequences based on quantitative structure and activity relationship (QSAR) was performed. This approach might represent a useful classification tool regarding health-related properties prior to further purification.
用来自短乳杆菌和乳酸乳球菌的两种细胞外蛋白酶制剂对绵羊奶和牛奶中的酪蛋白进行水解。通过HPLC-MS/MS分析水解产物以鉴定肽段。无论酪蛋白来源如何，均可观察到菌株依赖性肽谱，并且可以通过计算确定这两种蛋白酶的特异性。在乳酸乳球菌和短乳杆菌的水解产物中均出现了在C末端产生苯丙氨酸、亮氨酸或酪氨酸的切割模式。然而，短乳杆菌蛋白酶更倾向于在赖氨酸的C末端进行切割。水解产物显示出ACE抑制活性，IC值在16-70μg/mL范围内。绵羊酪蛋白水解产物具有更高的ACE抑制活性。在这些绵羊和牛奶酪蛋白水解产物中发现了先前描述的降压和阿片样肽，并基于定量结构-活性关系（QSAR）对序列的降压活性进行了预测。在进一步纯化之前，这种方法可能是一种关于健康相关特性的有用分类工具。
