Third Xiangya Hospital, Central South University, Changsha, China.
Third Xiangya Hospital, Central South University, Changsha, China.
EBioMedicine. 2017 Oct;24:277-285. doi: 10.1016/j.ebiom.2017.09.024. Epub 2017 Sep 23.
To assess the effect of storage time and temperature on complete blood count (CBC) and comprehensive metabolic panel (CMP) testing.
PubMed, EMBASE, the Cochrane Library of Systematic Reviews, Web of Science (WOS), China National Knowledge Infrastructure (CNKI), WanFang databases and SinoMed databases were searched up to May 2017. Clinical trials with adult whole blood samples were identified. Paired reviewers independently screened, extracted data and evaluated the quality of evidence (MINORS tool). Analyses were conducted using Revman 5.3 and Stata 14.0.
A total of 89 studies were confirmed. For CBC, except MPV, most parameters were stable at least for 24h. Some indices, such as WBC, PLt, HCT, HGB and MCH were stable up to 3 d. However, stable CMP test results could only be acquired within 12h. at 4°C, including GLU, AST, ALT, Na, ALB, Cl, DBIL, TC, TG and ALP. Values were less stable when stored at RT.
Specimens stored >12h. for CMP may generate unreliable results. For CBC, samples could reliably be stored for 24h. For longer storage, refrigeration (at 4°C) would be a better choice.
评估储存时间和温度对全血细胞计数（CBC）和综合代谢面板（CMP）检测的影响。
检索PubMed、EMBASE、Cochrane 系统评价图书馆、Web of Science（WOS）、中国国家知识基础设施（CNKI）、万方数据库和中国生物医学文献数据库（SinoMed），检索时间截至 2017 年 5 月。纳入成人全血样本的临床试验。由两名评价员独立筛选、提取资料和评价证据质量（MINORS 工具）。采用 RevMan 5.3 和 Stata 14.0 进行分析。
共确定 89 项研究。对于 CBC，除了 MPV，大多数参数至少在 24 h 内是稳定的。一些指标，如 WBC、PLt、HCT、HGB 和 MCH，在 3 d 内是稳定的。然而，只有在 4°C 下，CMP 检测结果在 12 h 内是稳定的，包括 GLU、AST、ALT、Na、ALB、Cl、DBIL、TC、TG 和 ALP。在 RT 下储存时，数值稳定性较差。
CMP 标本储存超过 12 h 可能会产生不可靠的结果。对于 CBC，标本可在 24 h 内可靠储存。如需更长时间储存，冷藏（4°C）是更好的选择。
