Laboratoire Suisse d'Analyse du Dopage, Centre Universitaire Romand de Médecine Légale, Centre Hospitalier Universitaire Vaudois, University of Lausanne, Epalinges, Switzerland.
Int J Lab Hematol. 2011 Apr;33(2):146-53. doi: 10.1111/j.1751-553X.2010.01256.x. Epub 2010 Aug 16.
With the setting up of the newly Athlete's Biological Passport antidoping programme, novel guidelines have been introduced to guarantee results beyond reproach. We investigated in this context, the effect of storage time on the variables commonly measured for the haematological passport. We also wanted to assess for these variables, the within and between analyzer variations.
Blood samples were obtained from top level male professional cyclists (27 samples for the first part of the study and 102 for the second part) taking part to major stage races. After collection, they were transported under refrigerated conditions (2 °C < T < 12 °C), delivered to the antidoping laboratory, analysed and then stored at approximately 4 °C to conduct analysis at different time points up to 72 h after delivery. A mixed-model procedure was used to determine the stability of the different variables.
As expected haemoglobin concentration was not affected by storage and showed stability for at least 72 h. Under the conditions of our investigation, the reticulocytes percentage showed a much better stability than previous published data (> 48 h) and the technical comparison of the haematology analyzer demonstrated excellent results.
In conclusion, our data clearly demonstrate that as long as the World Anti-Doping Agency's guidelines are followed rigorously, all blood results reach the quality level required in the antidoping context.
随着新的运动员生物护照反兴奋剂计划的设立，为了保证结果无可指责，引入了新的指导方针。在这种情况下，我们研究了储存时间对血液护照常用变量的影响。我们还想评估这些变量的内在和外在分析器变化。
从参加重大阶段比赛的顶级男性职业自行车手（第一部分 27 个样本，第二部分 102 个样本）中采集血液样本。采集后，它们在冷藏条件下（2°C<T<12°C）运输，送到反兴奋剂实验室进行分析，然后储存在大约 4°C的温度下，以在交付后 72 小时内进行不同时间点的分析。使用混合模型程序来确定不同变量的稳定性。
如预期的那样，血红蛋白浓度不受储存的影响，至少在 72 小时内保持稳定。在我们调查的条件下，网织红细胞百分比显示出比以前发表的数据（>48 小时）更好的稳定性，并且血液学分析仪的技术比较显示出优异的结果。
总之，我们的数据清楚地表明，只要严格遵守世界反兴奋剂机构的指导方针，所有血液检测结果都达到了反兴奋剂背景下所需的质量水平。
