Department of Biomaterials, Radboud University Medical Centre, 6500 HB, Nijmegen, The Netherlands.
National Dental Centre Singapore, 5 Second Hospital Ave, 168938, Singapore.
Nanomedicine (Lond). 2017 Nov;12(22):2771-2785. doi: 10.2217/nnm-2017-0172. Epub 2017 Oct 2.
To evaluate the antibacterial potential and biological performance of silver nanoparticles in chitosan-based membranes.
MATERIALS & METHODS: Electrospun chitosan/poly(ethylene oxide) membranes with different amounts of silver nanoparticles were evaluated for antibacterial properties and cytotoxicity in vitro and for tissue response in a rabbit subcutaneous model.
The nanoparticles displayed dose-dependent antibacterial properties against Porphyromonas gingivalis and Fusobacterium nucleatum, without showing noticeable cytotoxicity. The membranes with silver nanoparticles evoked a similar inflammatory response compared with the membranes without silver nanoparticles.
The antibacterial effect, combined with the findings on cyto- and biocompatibility warrants further investigation to the usefulness of chitosan/poly(ethylene oxide) membranes with silver nanoparticles, for clinical applications like guided tissue regeneration.
评估壳聚糖基膜中银纳米粒子的抗菌潜力和生物学性能。
评估了不同载银量的静电纺丝壳聚糖/聚氧化乙烯膜的体外抗菌性能和细胞毒性，以及在兔皮下模型中的组织反应。
纳米粒子对牙龈卟啉单胞菌和伴放线放线杆菌表现出剂量依赖性的抗菌作用，同时没有明显的细胞毒性。载银纳米粒子的膜与不含银纳米粒子的膜引起的炎症反应相似。
抗菌效果结合细胞毒性和生物相容性的发现，证明了载银壳聚糖/聚氧化乙烯膜在临床应用（如引导组织再生）中的潜在用途。
