Department of Chemistry and Technology of Polymers, Cracow University of Technology, Warszawska 24, 31-155 Krakow, Poland.
Institute of Inorganic Chemistry and Technology, Cracow University of Technology, Warszawska 24, 31-155 Krakow, Poland.
Colloids Surf B Biointerfaces. 2017 Dec 1;160:325-330. doi: 10.1016/j.colsurfb.2017.09.044. Epub 2017 Sep 20.
Chitosan based hydrogels are commonly applied in various fields of medicine and pharmacy. Modification of hydrogel polymers using nanosilver particles may result in formation of materials with enhanced antibacterial properties. In this article synthesis of hydrogel materials based on chitosan and modified with silver nanoparticles is presented. First, preparation and characterization of silver nanoparticles using UV-vis spectroscopy has been shown. Hydrogels modified with nanosilver particles were subjected to the measurements of swelling ability and in vitro tests in distilled water and Simulated Body Fluid (SBF), respectively. Additionally, evaluation of antibacterial properties against Staphylococcus aureus and Enterococcus faecalis as well as results of cytotoxicity of hydrogel materials modified with silver nanoparticles conducted by means of XTT and MTT assays using dermis cells BJ (CRL-2522TM) have been presented.
壳聚糖基水凝胶通常应用于医学和药学的各个领域。通过使用纳米银颗粒对水凝胶聚合物进行修饰，可能会形成具有增强的抗菌性能的材料。本文介绍了基于壳聚糖并通过纳米银颗粒修饰的水凝胶材料的合成。首先，通过紫外-可见光谱法展示了银纳米颗粒的制备和表征。对载纳米银颗粒的水凝胶进行了溶胀能力和在蒸馏水及模拟体液（SBF）中的体外测试。此外，还通过 XTT 和 MTT 测定法使用真皮细胞 BJ（CRL-2522TM）评估了对金黄色葡萄球菌和粪肠球菌的抗菌性能以及载纳米银颗粒的水凝胶材料的细胞毒性的结果。
