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长链非编码RNA-p21的下调通过不依赖Hippo的YAP激活促进胃癌发展。

Down regulation of lincRNA-p21 contributes to gastric cancer development through Hippo-independent activation of YAP.

作者信息

Chen Ying, Wei Guoqing, Xia Hongwei, Yu Huangfei, Tang Qiuling, Bi Feng

机构信息

Department of Medical Oncology, West China Hospital, Sichuan University, Chengdu, P.R. China.

State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, P.R. China.

出版信息

Oncotarget. 2017 Jul 10;8(38):63813-63824. doi: 10.18632/oncotarget.19130. eCollection 2017 Sep 8.

DOI:10.18632/oncotarget.19130
PMID:28969031
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5609963/
Abstract

Long intergenic non-coding RNA p21 (lincRNA-p21), known as the direct transcriptional target of p53, was found down-regulated in several human solid tumors. However, little is known about the role of lincRNA-p21 in gastric cancer. The expression levels of lincRNA-p21 in tissue samples and cell lines were detected by qRT-PCR. MGC-803 and MKN-45 cells were transfected with siRNAs targeting lincRNA-p21 or control siRNAs to determine the effect of reduced lincRNA-p21 expression on tumorigenesis. We also overexpressed lincRNA-p21 in MGC-803 cells. Cell proliferation was measured by CCK-8 and Ethynyl-2-deoxyuridine (EdU) incorporation assays. Migration and invasion abilities of cells were measured by wound healing and transwell assay. We demonstrated that lincRNA-p21 was significantly reduced in gastric cancer tissues (p<0.001) compared with that in normal tissues and this lower level of lincRNA-p21 was significantly correlated with higher invasion depth grade (p=0.024), more distant metastasis (p=0.009) and advanced TNM stage (p=0.011). Further study revealed that knock down of lincRNA-p21 could promote malignant behavior of gastric cancer cells and induce epithelial to mesenchymal transition (EMT). Overexpressing lincRNA-p21 showed opposite effects. Moreover, knocking down lincRNA-p21 could elevate the expression of Yes associated protein (YAP), the core effector of Hippo signaling, by elevating mRNA levels and increasing its nucleus translocation instead of the canonical Hippo pathway. Overexpression experiments verified the regulation role of lincRNA-p21 in YAP expression. Collectively, these data suggest that lincRNA-p21 could serve as a potential biomarker and a vital therapeutic target in gastric cancer.

摘要

长链基因间非编码RNA p21（lincRNA-p21）是p53的直接转录靶点，在多种人类实体瘤中被发现表达下调。然而，关于lincRNA-p21在胃癌中的作用知之甚少。通过qRT-PCR检测组织样本和细胞系中lincRNA-p21的表达水平。用靶向lincRNA-p21的小干扰RNA（siRNA）或对照siRNA转染MGC-803和MKN-45细胞，以确定lincRNA-p21表达降低对肿瘤发生的影响。我们还在MGC-803细胞中过表达lincRNA-p21。通过CCK-8和乙炔基-2-脱氧尿苷（EdU）掺入试验检测细胞增殖。通过伤口愈合试验和Transwell试验检测细胞的迁移和侵袭能力。我们证明，与正常组织相比，胃癌组织中lincRNA-p21显著降低（p<0.001），且lincRNA-p21的低水平与更高的浸润深度分级（p=0.024）、更多远处转移（p=0.009）和更高的TNM分期（p=0.011）显著相关。进一步研究表明，敲低lincRNA-p21可促进胃癌细胞的恶性行为并诱导上皮-间质转化（EMT）。过表达lincRNA-p21则显示出相反的效果。此外，敲低lincRNA-p21可通过提高mRNA水平并增加其核转位而非经典的Hippo信号通路来提高Yes相关蛋白（YAP）的表达，YAP是Hippo信号的核心效应分子。过表达实验验证了lincRNA-p21对YAP表达的调控作用。总体而言，这些数据表明lincRNA-p21可能是胃癌的潜在生物标志物和重要治疗靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c72/5609963/5bce96bf1eba/oncotarget-08-63813-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c72/5609963/1465c1e57657/oncotarget-08-63813-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c72/5609963/94ff4679b5d1/oncotarget-08-63813-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c72/5609963/12495fd69e92/oncotarget-08-63813-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c72/5609963/933dbc2c1add/oncotarget-08-63813-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c72/5609963/84854faf72aa/oncotarget-08-63813-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c72/5609963/fbd4c5d139f7/oncotarget-08-63813-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c72/5609963/5bce96bf1eba/oncotarget-08-63813-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c72/5609963/1465c1e57657/oncotarget-08-63813-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c72/5609963/94ff4679b5d1/oncotarget-08-63813-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c72/5609963/12495fd69e92/oncotarget-08-63813-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c72/5609963/933dbc2c1add/oncotarget-08-63813-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c72/5609963/84854faf72aa/oncotarget-08-63813-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c72/5609963/fbd4c5d139f7/oncotarget-08-63813-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c72/5609963/5bce96bf1eba/oncotarget-08-63813-g007.jpg

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本文引用的文献

1
Cancer statistics in China, 2015.《中国癌症统计数据 2015》
CA Cancer J Clin. 2016 Mar-Apr;66(2):115-32. doi: 10.3322/caac.21338. Epub 2016 Jan 25.
2
LincRNA-p21 activates endoplasmic reticulum stress and inhibits hepatocellular carcinoma.长链非编码RNA-p21激活内质网应激并抑制肝细胞癌。
Oncotarget. 2015 Sep 29;6(29):28151-63. doi: 10.18632/oncotarget.4661.
3
HOTAIR: an oncogenic long non-coding RNA in different cancers.HOTAIR：一种在不同癌症中发挥致癌作用的长链非编码RNA
长链非编码RNA（lncRNAs）在HIV介导的致癌作用中的作用：在细胞稳态、细胞存活过程及耐药性中的作用
Noncoding RNA Res. 2022 Jul 31;7(3):184-196. doi: 10.1016/j.ncrna.2022.07.003. eCollection 2022 Sep.
4
The Epithelial-Mesenchymal Transition at the Crossroads between Metabolism and Tumor Progression.上皮-间充质转化在代谢与肿瘤进展的交汇点。
Int J Mol Sci. 2022 Jan 12;23(2):800. doi: 10.3390/ijms23020800.
5
Conservation of Epithelial-to-Mesenchymal Transition Process in Neural Crest Cells and Metastatic Cancer.神经嵴细胞和转移性癌症中上皮-间充质转化过程的保守性。
Cells Tissues Organs. 2021;210(3):151-172. doi: 10.1159/000516466. Epub 2021 Jul 2.
6
PKCδ-mediated SGLT1 upregulation confers the acquired resistance of NSCLC to EGFR TKIs.PKCδ 介导的 SGLT1 上调赋予 NSCLC 对 EGFR TKI 的获得性耐药性。
Oncogene. 2021 Jul;40(29):4796-4808. doi: 10.1038/s41388-021-01889-0. Epub 2021 Jun 21.
7
Cisatracurium inhibits the growth and induces apoptosis of ovarian cancer cells by promoting lincRNA-p21.顺式阿曲库铵通过促进 lincRNA-p21 抑制卵巢癌细胞的生长并诱导其凋亡。
Bioengineered. 2021 Dec;12(1):1505-1516. doi: 10.1080/21655979.2021.1916271.
8
Advances in Understanding the LncRNA-Mediated Regulation of the Hippo Pathway in Cancer.癌症中长链非编码RNA介导的Hippo信号通路调控机制的研究进展
Onco Targets Ther. 2021 Apr 7;14:2397-2415. doi: 10.2147/OTT.S283157. eCollection 2021.
9
Long Non-Coding RNA THOR Enhances the Stem Cell-Like Traits of Triple-Negative Breast Cancer Cells Through Activating β-Catenin Signaling.长非编码 RNA THOR 通过激活β-catenin 信号增强三阴性乳腺癌细胞的干细胞样特征。
Med Sci Monit. 2020 Jul 14;26:e923507. doi: 10.12659/MSM.923507.
10
An update on long intergenic noncoding RNA p21: a regulatory molecule with various significant functions in cancer.长链基因间非编码RNA p21的最新进展：一种在癌症中具有多种重要功能的调控分子
Cell Biosci. 2020 Jun 22;10:82. doi: 10.1186/s13578-020-00445-9. eCollection 2020.
Cancer Biol Med. 2015 Mar;12(1):1-9. doi: 10.7497/j.issn.2095-3941.2015.0006.
4
Long noncoding RNA MALAT-1 enhances stem cell-like phenotypes in pancreatic cancer cells.长链非编码RNA MALAT-1增强胰腺癌细胞中的干细胞样表型。
Int J Mol Sci. 2015 Mar 24;16(4):6677-93. doi: 10.3390/ijms16046677.
5
Long-term survival after endoscopic resection versus surgery in early gastric cancers.早期胃癌内镜切除与手术治疗后的长期生存率比较
Endoscopy. 2015 Apr;47(4):293-301. doi: 10.1055/s-0034-1391284. Epub 2015 Jan 27.
6
Alteration of epithelial-mesenchymal transition markers in human normal ovaries and neoplastic ovarian cancers.人类正常卵巢和卵巢癌组织中上皮-间充质转化标志物的改变。
Int J Oncol. 2015 Jan;46(1):272-80. doi: 10.3892/ijo.2014.2695. Epub 2014 Oct 7.
7
Cancer incidence and mortality worldwide: sources, methods and major patterns in GLOBOCAN 2012.全球癌症发病与死亡：GLOBOCAN 2012 数据源、方法与主要模式。
Int J Cancer. 2015 Mar 1;136(5):E359-86. doi: 10.1002/ijc.29210. Epub 2014 Oct 9.
8
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Arthritis Rheumatol. 2014 Nov;66(11):2947-57. doi: 10.1002/art.38805.
9
YAP/TAZ incorporation in the β-catenin destruction complex orchestrates the Wnt response.YAP/TAZ 与 β-连环蛋白降解复合物的结合调控 Wnt 反应。
Cell. 2014 Jul 3;158(1):157-70. doi: 10.1016/j.cell.2014.06.013. Epub 2014 Jun 26.
10
Hippo pathway activity influences liver cell fate.河马通路活性影响肝细胞命运。
Cell. 2014 Jun 5;157(6):1324-1338. doi: 10.1016/j.cell.2014.03.060.