结核病与艾滋病毒/结核病合并感染中的氧化应激标志物
Oxidative Stress Markers in Tuberculosis and HIV/TB Co-Infection.
作者信息
Rajopadhye Shreewardhan Haribhau, Mukherjee Sandeepan R, Chowdhary Abhay S, Dandekar Sucheta P
机构信息
PhD Fellow, Department of Biochemistry, Seth G.S. Medical College and KEM Hospital and Department of Biochemistry, Haffkine Institute, Parel, Mumbai, Maharashtra, India.
Scientific Officer, Department of Virology and Immunology, Haffkine Institute, Parel, Mumbai, Maharashtra, India.
出版信息
J Clin Diagn Res. 2017 Aug;11(8):BC24-BC28. doi: 10.7860/JCDR/2017/28478.10473. Epub 2017 Aug 1.
INTRODUCTION
Dysfunction of redox homeostasis has been implicated in many pathological conditions. An imbalance of pro- and anti-oxidants have been observed in Tuberculosis (TB) and its co-morbidities especially HIV/AIDS. The pro inflammatory milieu in either condition aggravates the physiological balance of the redox mechanisms. The present study therefore focuses on assessing the redox status of patients suffering from TB and HIV-TB co-infection.
AIM
To assess the oxidative stress markers in the HIV-TB and TB study cohort.
MATERIALS AND METHODS
The current prospective study was conducted in Haffkine Institute, Parel, Maharashtra, India, during January 2013 to December 2015. Blood samples from 50 patients each suffering from active TB and HIV-TB co-infection were collected from Seth G.S.Medical College and KEM Hospital Mumbai and Group of Tuberculosis Hospital, Sewree Mumbai. Samples were processed and the experiments were carried out at the Department of Biochemistry, Haffkine Institute. Samples from 50 healthy volunteers were used as controls. Serum was assessed for pro-oxidant markers such as Nitric Oxide (NO), Thiobarbituric Acid Reactive Species (TBARS), C-Reactive Protein (CRP), superoxide anion. Antioxidant markers such as catalase and Superoxide Dismutase (SOD) were assessed. Total serum protein, was also assessed.
RESULTS
Among the pro-oxidants, serum NO levels were decreased in TB group while no change was seen in HIV-TB group. TBARS and CRP levels showed significant increase in both groups; superoxide anion increased significantly in HIV-TB group. Catalase levels showed decreased activities in TB group. SOD activity significantly increased in HIV-TB but not in TB group. The total serum proteins were significantly increased in HIV-TB and TB groups. The values of Control cohort were with the normal reference ranges.
CONCLUSION
In the present study, we found the presence of oxidative stress to be profound in the TB and HIV-TB co-infection population.
引言
氧化还原稳态功能障碍与许多病理状况有关。在结核病（TB）及其合并症尤其是艾滋病毒/艾滋病中，已观察到促氧化剂和抗氧化剂失衡。在这两种情况下，促炎环境都会加剧氧化还原机制的生理平衡。因此，本研究着重评估结核病患者和艾滋病毒-结核病合并感染患者的氧化还原状态。
目的
评估艾滋病毒-结核病和结核病研究队列中的氧化应激标志物。
材料与方法
本前瞻性研究于2013年1月至2015年12月在印度马哈拉施特拉邦帕雷尔的哈夫金研究所进行。从孟买的塞思·G.S.医学院和KEM医院以及孟买塞弗里结核病医院收集了50例活动性结核病患者和50例艾滋病毒-结核病合并感染患者的血样。样本经过处理后，实验在哈夫金研究所生物化学系进行。使用50名健康志愿者的样本作为对照。评估血清中的促氧化剂标志物，如一氧化氮（NO）、硫代巴比妥酸反应性物质（TBARS）、C反应蛋白（CRP）、超氧阴离子。评估抗氧化剂标志物，如过氧化氢酶和超氧化物歧化酶（SOD）。还评估了总血清蛋白。
结果
在促氧化剂中，结核病组血清NO水平降低，而艾滋病毒-结核病组未见变化。两组的TBARS和CRP水平均显著升高；艾滋病毒-结核病组超氧阴离子显著增加。结核病组过氧化氢酶水平活性降低。艾滋病毒-结核病组SOD活性显著增加，而结核病组未增加。艾滋病毒-结核病组和结核病组的总血清蛋白均显著增加。对照组的值在正常参考范围内。
结论
在本研究中，我们发现氧化应激在结核病和艾滋病毒-结核病合并感染人群中非常严重。
Zotero 插件