开发一种用于可视化卵巢癌细胞中β-半乳糖苷酶的长寿命荧光探针。
Development of a Long-Lived Luminescence Probe for Visualizing β-Galactosidase in Ovarian Carcinoma Cells.
机构信息
Department of Chemistry, Hong Kong Baptist University , Kowloon Tong, Hong Kong, China.
State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau , Macao, China.
出版信息
Anal Chem. 2017 Nov 7;89(21):11679-11684. doi: 10.1021/acs.analchem.7b03114. Epub 2017 Oct 12.
β-Galactosidase (β-gal) is an important biomarker for ovarian cancers. In this work, we designed and synthesized a novel iridium(III)-based probe 1 for discriminating ovarian carcinoma cell lines from normal cell lines. The probe could detect β-gal even in the presence of a highly autofluorescent background. The probe also showed a good linear response to β-gal between 0 and 30 U/mL, with a detection limit of 0.51 U/mL. Importantly, complex 1 could selectively "light up" ovarian carcinoma cells, while exhibiting negligible luminescence in normal cells. Overall, complex 1 could be potentially used as a useful probe for detecting β-gal expression in the context of ovarian cancer diagnostics.
β-半乳糖苷酶（β-gal）是卵巢癌的重要生物标志物。在这项工作中，我们设计并合成了一种新型的基于铱（III）的探针 1，用于区分卵巢癌细胞系和正常细胞系。该探针即使在存在高度自发荧光背景的情况下也能检测到β-gal。探针对 0 到 30 U/mL 之间的β-gal 具有良好的线性响应，检测限为 0.51 U/mL。重要的是，配合物 1 可以选择性地“点亮”卵巢癌细胞，而在正常细胞中几乎没有发光。总的来说，配合物 1 可以潜在地用作卵巢癌诊断中检测β-gal 表达的有用探针。
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