Wang Wanhe, Liu Jianhua, Kong Lingtan, Wang Ling, Niu Dou, Wang Jing, Leung Chung-Hang
Xi'an Key Laboratory of Stem Cell and Regenerative Medicine, Institute of Medical Research, Northwestern Polytechnical University, 127 West Youyi Road, Xi'an, 710072, Shaanxi, China.
Northwestern Polytechnical University Chongqing Technology Innovation Center, Chongqing, 400000, People's Republic of China.
Mikrochim Acta. 2023 Apr 5;190(5):171. doi: 10.1007/s00604-023-05762-7.
A new method is presented for the one-step synthesis and real-time monitoring of iridium(III) complex-functionalized AuNPs from the precursor gold(III) chloride (AuCl). The functionalized AuNPs with an average size of 8 - 20 nm were obtained by the reduction of Au ions by the alkyne group of iridium(III) complexes, which was accompanied by the anchoring iridium(III) complexes on the surface of the nanoparticles. Meanwhile, the luminescence of the iridium(III) complexes was effectively quenched due to distance-dependent fluorescence quenching by AuNPs, thereby enabling luminescence monitoring of the formation process of the functionalized AuNPs and obtaining scattering information and spectral information in real time. Moreover, this method was applied to the determination of Au ions in buffer with a limit of detection of 0.38 μM at 700 nm in luminescence mode, while the detection limit for absorbance was 10.04 μM. Importantly, the multimodal detection strategy alleviates interference from other metal ions. Furthermore, the iridium(III) alkyne complexes were capable of imaging mitochondrial Au ions in living cells. Taken together, this work opens a new avenue for convenient synthesis and monitoring formation of functionalized AuNPs, and also provides a tool for selective determination of Au ions in solution and in cellulo.
本文提出了一种新方法，可从前体氯化金（AuCl）一步合成并实时监测铱（III）配合物功能化的金纳米颗粒（AuNPs）。通过铱（III）配合物的炔基还原金离子，获得了平均尺寸为8 - 20 nm的功能化AuNPs，同时铱（III）配合物锚定在纳米颗粒表面。与此同时，由于AuNPs的距离依赖性荧光猝灭，铱（III）配合物的发光被有效猝灭，从而能够对功能化AuNPs的形成过程进行发光监测，并实时获取散射信息和光谱信息。此外，该方法应用于缓冲液中Au离子的测定，在发光模式下700 nm处的检测限为0.38 μM，而吸光度检测限为10.04 μM。重要的是，多模态检测策略减轻了其他金属离子的干扰。此外，铱（III）炔基配合物能够对活细胞中的线粒体Au离子进行成像。综上所述，这项工作为功能化AuNPs的便捷合成和形成监测开辟了一条新途径，也为溶液和细胞中Au离子的选择性测定提供了一种工具。
