Department of Cellular Biology & Anatomy, Louisiana State University Health Sciences Center-Shreveport, Shreveport, LA, USA.
Basic Biomedical Sciences, Sanford School of Medicine The University of South Dakota, Vermillion, SD, USA.
Sci Rep. 2017 Oct 2;7(1):12547. doi: 10.1038/s41598-017-12720-w.
Increasing evidence suggest that low-dose alcohol consumption (LAC) reduces the incidence and improves the functional outcome of ischemic stroke. We determined the influence of LAC on post-ischemic inflammation. Male Sprague-Dawley rats were divided into 3 groups, an ethanol (13.5% alcohol) group, a red wine (Castle Rock Pinot Noir, 13.5% alcohol) group, and a control group. The amount of alcohol given to red wine and ethanol groups was 1.4 g/kg/day. After 8 weeks, the animals were subjected to a 2-hour middle cerebral artery occlusion (MCAO) and sacrificed at 24 hours of reperfusion. Cerebral ischemia/reperfusion (I/R) injury, expression of adhesion molecules and pro- and anti-inflammatory cytokines/chemokines, microglial activation and neutrophil infiltration were evaluated. The total infarct volume and neurological deficits were significantly reduced in red wine- and ethanol-fed rats compared to control rats. Both red wine and ethanol suppressed post-ischemic expression of adhesion molecules and microglial activation. In addition, both red wine and ethanol upregulated expression of tissue inhibitor of metalloproteinases 1 (TIMP-1), downregulated expression of proinflammatory cytokines/chemokines, and significantly alleviated post-ischemic expression of inflammatory mediators. Furthermore, red wine significantly reduced post-ischemic neutrophil infiltration. Our findings suggest that LAC may protect the brain against its I/R injury by suppressing post-ischemic inflammation.
越来越多的证据表明，低剂量饮酒（LAC）可降低缺血性中风的发病率并改善其功能预后。我们确定了 LAC 对缺血后炎症的影响。雄性 Sprague-Dawley 大鼠分为 3 组，乙醇（13.5%酒精）组、红酒（Castle Rock Pinot Noir，13.5%酒精）组和对照组。给予红酒和乙醇组的酒精量为 1.4 g/kg/天。8 周后，动物进行 2 小时大脑中动脉闭塞（MCAO），再灌注 24 小时后处死。评估脑缺血/再灌注（I/R）损伤、黏附分子和促炎及抗炎细胞因子/趋化因子的表达、小胶质细胞激活和中性粒细胞浸润。与对照组相比，红酒和乙醇喂养的大鼠的总梗死体积和神经功能缺损明显减少。红酒和乙醇均抑制缺血后黏附分子的表达和小胶质细胞的激活。此外，红酒和乙醇均上调组织金属蛋白酶抑制剂 1（TIMP-1）的表达，下调促炎细胞因子/趋化因子的表达，并显著减轻缺血后炎症介质的表达。此外，红酒可显著减少缺血后中性粒细胞的浸润。我们的研究结果表明，LAC 可能通过抑制缺血后炎症来保护大脑免受 I/R 损伤。
