Yin Xiao-Lu, Liu Hua-Yu, Zhang Yu-Qing
Silk Biotechnology Laboratory, School of Biology and Basic Medical Sciences, Soochow University, Suzhou, P R China.
National Engineering Laboratory for Modern Silk, Soochow University, Suzhou, P R China.
Food Nutr Res. 2017 Sep 6;61(1):1368847. doi: 10.1080/16546628.2017.1368847. eCollection 2017.
This experiment, based on the previous study on , introduces whole mulberry branch powder into the diet to treat diabetic mice. Mulberry branch bark powder (MBBP) was administered orally to streptozotocin (STZ)-induced type II diabetic (T2D) mice to investigate hypoglycemic effects. After a 4-week period of diet consumption containing 5%, 10% and 20% MBBP, the fasting blood glucose, body weight and the related western blotting were measured, pathologic and immunohistochemical were observed. The 20% MBBP group showed a significant reduction in hyperglycemia and hyperinsulinemia; fasting blood glucose and insulin decreased from 25.0 to 14.8 mmol/L and 26.5 to 16.0 mU/L, respectively. Pathologic and immunohistochemical observation showed that MBBP administration lead to the repair of pancreas cells and restoration of insulin secretion. Dietary MBBP was associated with the decrease in the contents of 3, 4-methylenedioxeamphetamine, 8-OHdG, aspartate aminotransferase, and alanine aminotransferase, and the increase in antioxidative ability and glucose tolerance. Western blotting (WB) analysis suggested that MBBP decreased the TNF-α levels, thus relieving inflammation and improving liver function. It also led to the downregulation of apoptosis factor expression. WB also confirmed that MBBP enhanced the gene expression of the key enzymes: insulin receptor, insulin receptor substrate, glycogen synthase, G6Pase and phosphoenolpyruvate carboxykinase, which are related to glucose metabolism in the liver, and increase the expression of the genes and glucokinase, related to insulin secretion. Thus, oral administration of MBBP regulated insulin secretion and effectively maintained normal levels of glucose metabolism in mice, which may be done by improving the antioxidant capacity and activating insulin signaling with T2D..
本实验基于之前关于……的研究，将全桑枝粉引入饮食中以治疗糖尿病小鼠。将桑枝皮粉（MBBP）口服给予链脲佐菌素（STZ）诱导的II型糖尿病（T2D）小鼠，以研究其降血糖作用。在食用含5%、10%和20% MBBP的饮食4周后，测量空腹血糖、体重并进行相关的蛋白质免疫印迹分析，观察病理和免疫组化情况。20% MBBP组的高血糖和高胰岛素血症显著降低；空腹血糖和胰岛素分别从25.0 mmol/L降至14.8 mmol/L以及从26.5 mU/L降至16.0 mU/L。病理和免疫组化观察表明，给予MBBP可导致胰腺细胞修复和胰岛素分泌恢复。饮食中的MBBP与3,4-亚甲基二氧甲基苯丙胺、8-羟基脱氧鸟苷、天冬氨酸转氨酶和丙氨酸转氨酶含量的降低以及抗氧化能力和葡萄糖耐量的增加有关。蛋白质免疫印迹（WB）分析表明，MBBP降低了TNF-α水平，从而减轻炎症并改善肝功能。它还导致凋亡因子表达下调。WB还证实，MBBP增强了与肝脏葡萄糖代谢相关的关键酶：胰岛素受体、胰岛素受体底物、糖原合酶、葡萄糖-6-磷酸酶和磷酸烯醇式丙酮酸羧激酶的基因表达，并增加了与胰岛素分泌相关的基因……和葡萄糖激酶的表达。因此，口服MBBP可调节胰岛素分泌并有效维持小鼠葡萄糖代谢的正常水平，这可能是通过提高抗氧化能力和激活T2D的胰岛素信号来实现的。
