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牛产肠毒素大肠杆菌中编码F17菌毛结构蛋白的F17 - A基因的分离与核苷酸序列分析

Isolation and nucleotide sequence of the F17-A gene encoding the structural protein of the F17 fimbriae in bovine enterotoxigenic Escherichia coli.

作者信息

Lintermans P, Pohl P, Deboeck F, Bertels A, Schlicker C, Vandekerckhove J, Van Damme J, Van Montagu M, De Greve H

机构信息

Laboratory of Bacteriology, National Institute for Veterinary Research, Brussels, Belgium.

出版信息

Infect Immun. 1988 Jun;56(6):1475-84. doi: 10.1128/iai.56.6.1475-1484.1988.

DOI:10.1128/iai.56.6.1475-1484.1988
PMID:2897333
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC259424/
Abstract

The genetic determinant for production of the fimbrial F17 adhesive antigen was isolated from a bovine enterotoxigenic Escherichia coli strain. The F17-A gene, coding for the structural component of the F17 fimbrial adhesin, was cloned and sequenced. An open reading frame of 540 base pairs encoding a polypeptide of 180 amino acids, of which the NH2-terminal 21 residues are characteristic of a signal sequence, has been characterized. The mature protein lacks histidine, methionine, and tryptophan. A possible promoter and ribosome binding site as well as a possible site for termination of transcription are proposed. An important homology of the F17-A protein with fimA and papA fimbrial proteins was found. The N-terminal sequence of the mature F17-A pilin is extremely similar to the N-terminal sequence of the G fimbriae identified on human pyelonephritogenic E. coli strains.

摘要

从一株牛源产肠毒素大肠杆菌中分离出了编码菌毛F17黏附抗原的遗传决定因子。对编码F17菌毛黏附素结构成分的F17 - A基因进行了克隆和测序。已鉴定出一个540个碱基对的开放阅读框，其编码一个180个氨基酸的多肽，其中氨基末端的21个残基具有信号序列的特征。成熟蛋白不含组氨酸、甲硫氨酸和色氨酸。提出了一个可能的启动子和核糖体结合位点以及一个可能的转录终止位点。发现F17 - A蛋白与fimA和papA菌毛蛋白有重要的同源性。成熟F17 - A菌毛蛋白的N末端序列与在人肾盂肾炎源大肠杆菌菌株上鉴定出的G菌毛的N末端序列极其相似。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a47/259424/348a9be0ce99/iai00078-0082-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a47/259424/e71840c635f3/iai00078-0078-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a47/259424/e1c188fb4e99/iai00078-0079-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a47/259424/51426a5af6a9/iai00078-0080-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a47/259424/dfc2fdb15c29/iai00078-0081-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a47/259424/348a9be0ce99/iai00078-0082-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a47/259424/e71840c635f3/iai00078-0078-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a47/259424/e1c188fb4e99/iai00078-0079-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a47/259424/51426a5af6a9/iai00078-0080-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a47/259424/dfc2fdb15c29/iai00078-0081-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a47/259424/348a9be0ce99/iai00078-0082-a.jpg

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