Charng Jason, Tan Rose, Luu Chi D, Sadigh Sam, Stambolian Dwight, Guymer Robyn H, Jacobson Samuel G, Cideciyan Artur V
Scheie Eye Institute, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, United States.
Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital, East Melbourne, Victoria, Australia.
Invest Ophthalmol Vis Sci. 2017 Oct 1;58(12):4940-4947. doi: 10.1167/iovs.17-22540.
To evaluate whether a practical method of imaging lenticular autofluorescence (AF) can provide an individualized measure correlated with age-related lens yellowing in older subjects undergoing tests involving shorter wavelength lights.
Lenticular AF was imaged with 488-nm excitation using a confocal scanning laser ophthalmoscope (cSLO) routinely used for retinal AF imaging. There were 75 older subjects (ages 47-87) at two sites; a small cohort of younger subjects served as controls. At one site, the cSLO was equipped with an internal reference to allow quantitative AF measurements; at the other site, reduced-illuminance AF imaging (RAFI) was used. In a subset of subjects, lens density index was independently estimated from dark-adapted spectral sensitivities performed psychophysically.
Lenticular AF intensity was significantly higher in the older eyes than the younger cohort when measured with the internal reference (59.2 ± 15.4 vs. 134.4 ± 31.7 gray levels; P < 0.05) as well as when recorded with RAFI without the internal reference (10.9 ± 1.5 vs. 26.1 ± 5.7 gray levels; P < 0.05). Lenticular AF was positively correlated with age; however, there could also be large differences between individuals of similar age. Lenticular AF intensity correlated well with lens density indices estimated from psychophysical measures.
Lenticular AF measured with a retinal cSLO can provide a practical and individualized measure of lens yellowing, and may be a good candidate to distinguish between preretinal and retinal deficits involving short-wavelength lights in older eyes.
评估一种实用的晶状体自发荧光（AF）成像方法是否能提供与接受涉及较短波长光测试的老年受试者年龄相关晶状体变黄相关的个体化测量指标。
使用常规用于视网膜AF成像的共焦扫描激光检眼镜（cSLO），以488nm激发光对晶状体AF进行成像。在两个地点有75名老年受试者（年龄47 - 87岁）；一小群年轻受试者作为对照。在一个地点，cSLO配备了内部参考以进行定量AF测量；在另一个地点，使用了低照度AF成像（RAFI）。在一部分受试者中，通过心理物理学方法从暗适应光谱敏感度独立估计晶状体密度指数。
当使用内部参考测量时，老年受试者眼睛的晶状体AF强度显著高于年轻受试者队列（59.2±15.4对134.4±31.7灰度级；P<0.05），以及在没有内部参考的情况下用RAFI记录时（10.9±1.5对26.1±5.7灰度级；P<0.05）。晶状体AF与年龄呈正相关；然而，相似年龄的个体之间也可能存在很大差异。晶状体AF强度与通过心理物理学测量估计的晶状体密度指数相关性良好。
用视网膜cSLO测量的晶状体AF可以提供一种实用且个体化的晶状体变黄测量方法，并且可能是区分老年眼中涉及短波长光的视网膜前和视网膜缺陷的良好候选指标。
