Department of Ophthalmology and Visual Sciences, School of Medicine, University of Alabama at Birmingham, Birmingham, AL, USA.
University Hospital Würzburg, Würzburg, Germany.
Transl Vis Sci Technol. 2022 Oct 3;11(10):23. doi: 10.1167/tvst.11.10.23.
The purpose of this study was to investigate quantitative autofluorescence (qAF8) in patients with and without early or intermediate age-related macular degeneration (AMD); to determine the impact of the aged crystalline lens and posterior capsular opacification (PCO).
In phakic and pseudophakic eyes ≥60 years, AMD status was determined by the Beckman system. PCO presence and severity was extracted from clinical records. qAF8 was calculated using custom FIJI plugins. Differences in qAF8, stratified by lens status, PCO severity, and AMD status, were analyzed using generalized estimating equations.
In 210 eyes of 115 individuals (mean age = 75.7 ± 6.6 years), qAF8 was lower in intermediate AMD compared to early AMD (P = 0.05). qAF8 did not differ between phakic and pseudophakic eyes (P = 0.8909). In phakic (n = 83) and pseudophakic (n = 127) eyes considered separately, qAF8 did not differ by AMD status (P = 0.0936 and 0.3494, respectively). Qualitative review of qAF images in phakic eyes illustrated high variability. In pseudophakic eyes, qAF8 did not differ with PCO present versus absent (54.5% vs. 45.5%). Review of implanted intraocular lenses (IOLs) revealed that 43.9% were blue-filter IOLs.
qAF8 was not associated with AMD status, up to intermediate AMD, considering only pseudophakic eyes to avoid noisy images in phakic eyes. In pseudophakic eyes, qAF8 was not affected by PCO. Because blue-filter IOLs may reduce levels of exciting light for qAF8, future studies investigating qAF in eyes with different IOL types are needed.
To reduce variability in observational studies and clinical trials requiring qAF8, pseudophakic participants without blue-filter IOLs or advanced PCO should be preferentially enrolled.
本研究旨在探讨伴有或不伴早期或中期年龄相关性黄斑变性（AMD）的患者的定量自发荧光（qAF8）；确定老化晶状体和后囊混浊（PCO）的影响。
在≥60 岁的有晶状体和无晶状体眼中，通过贝克曼系统确定 AMD 状态。从临床记录中提取 PCO 存在和严重程度。使用定制的 FIJI 插件计算 qAF8。使用广义估计方程分析按晶状体状态、PCO 严重程度和 AMD 状态分层的 qAF8 差异。
在 115 名个体的 210 只眼中（平均年龄 75.7 ± 6.6 岁），与早期 AMD 相比，中期 AMD 的 qAF8 较低（P = 0.05）。有晶状体和无晶状体眼之间的 qAF8 无差异（P = 0.8909）。在单独考虑的有晶状体（n = 83）和无晶状体（n = 127）眼中，qAF8 与 AMD 状态无差异（分别为 P = 0.0936 和 0.3494）。有晶状体眼中 qAF 图像的定性分析表明存在高度变异性。在无晶状体眼中，存在与不存在 PCO 的 qAF8 无差异（54.5%对 45.5%）。对植入的人工晶状体（IOL）的审查显示，43.9%为蓝滤光 IOL。
仅考虑无晶状体眼以避免有晶状体眼的图像噪声，在考虑到所有无晶状体眼的情况下，qAF8 与 AMD 状态无关，直到中期 AMD。在无晶状体眼中，qAF8 不受 PCO 影响。由于蓝滤光 IOL 可能降低 qAF8 的激发光水平，因此需要对具有不同 IOL 类型的眼睛进行 qAF 研究。
翻译后的中文文本与原文内容完全一致，没有任何篡改或歪曲。
