Agrawal Y P, Vanha-Perttula T
Department of Anatomy, University of Kuopio, Finland.
Int J Androl. 1988 Apr;11(2):123-31. doi: 10.1111/j.1365-2605.1988.tb00988.x.
The distribution of glutathione (GSH), L-glutamic acid (Glu) and gamma-glutamyl transpeptidase (gamma-GT) was studied in bull reproductive organs and fluids. Glutathione, the physiological substrate of gamma-GT, was localized specifically by a fluorescence method in the testis, epididymis and spermatozoa. Of the reproductive tissues, the testis, caput epididymis and ampulla had the highest levels of GSH, but it was also present in seminal fluid. Washed caput epididymal sperm had three times the GSH content of cauda epididymal or ejaculated sperm. In spermatozoa, GSH displayed maximal staining in the midpiece and tail regions. The highest levels of gamma-GT were encountered in the epididymis. The concentration of Glu was also high in the epididymis. Its formation may be due to the hydrolytic activity of gamma-GT, which, in addition, may have an important role in the transfer of Glu residues to reactive groups on the sperm surface.
研究了谷胱甘肽（GSH）、L-谷氨酸（Glu）和γ-谷氨酰转肽酶（γ-GT）在公牛生殖器官和体液中的分布。γ-GT的生理底物谷胱甘肽通过荧光法在睾丸、附睾和精子中特异性定位。在生殖组织中，睾丸、附睾头和壶腹的谷胱甘肽水平最高，但精液中也有谷胱甘肽。洗涤后的附睾头精子的GSH含量是附睾尾或射精精子的三倍。在精子中，GSH在中段和尾部区域显示出最大染色。γ-GT在附睾中的水平最高。附睾中Glu的浓度也很高。其形成可能归因于γ-GT的水解活性，此外，γ-GT可能在将Glu残基转移到精子表面的反应基团方面具有重要作用。
