Rizkallah Natalie, Chambers Caitlin G, de Graaf Simon P, Rickard Jessica P
School of Life and Environmental Sciences, Faculty of Science, University of Sydney, Sydney, NSW 2006, Australia.
Animals (Basel). 2022 Jan 20;12(3):244. doi: 10.3390/ani12030244.
Semen preservation is an essential component of reproductive technologies, as it promotes genetic gain and long-distance semen transport and multiplies the number of ewes able to be inseminated per single ejaculate. However, the reduced temperature during cold storage at 5 or 15 °C inflicts sub-lethal damage to spermatozoa, compromising sperm quality and the success of artificial breeding. New and emerging research in various species has reported the advantages of storing spermatozoa at higher temperatures, such as 23 °C; however, this topic has not been thoroughly investigated for ram spermatozoa. Despite the success of storing spermatozoa at 23 °C, sperm quality can be compromised by the damaging effects of lipid peroxidation, more commonly when metabolism is left unaltered during 23 °C storage. Additionally, given the biosafety concern surrounding the international transport of egg-yolk-containing extenders, further investigation is critical to assess the preservation ability of synthetic extenders and whether pro-survival factors could be supplemented to maximise sperm survival during storage at 23 °C.
精液保存是生殖技术的一个重要组成部分，因为它能促进遗传增益和长距离精液运输，并增加每次射精可授精母羊的数量。然而，在5或15°C冷藏期间温度降低会对精子造成亚致死损伤，损害精子质量和人工授精的成功率。不同物种的新出现的研究报告了在较高温度（如23°C）下储存精子的优点；然而，对于公羊精子，这个话题尚未得到充分研究。尽管在23°C下储存精子取得了成功，但脂质过氧化的破坏作用可能会损害精子质量，在23°C储存期间代谢保持不变时更常见。此外，鉴于围绕含蛋黄稀释液国际运输的生物安全问题，进一步研究对于评估合成稀释液的保存能力以及是否可以补充促存活因子以在23°C储存期间最大限度地提高精子存活率至关重要。
