构巢曲霉复杂AROM基因座的分离及核苷酸序列
The isolation and nucleotide sequence of the complex AROM locus of Aspergillus nidulans.
作者信息
Charles I G, Keyte J W, Brammar W J, Smith M, Hawkins A R
出版信息
Nucleic Acids Res. 1986 Mar 11;14(5):2201-13. doi: 10.1093/nar/14.5.2201.
Abstract
The AROM locus of A. nidulans, which governs five consecutive steps in pre-chorismate aromatic amino acid biosynthesis, has been cloned in a bacteriophage vector. The nucleotide sequence of the locus reveals a single, open reading-frame of 4,812 base-pairs, apparently without introns. An internal segment of the A. nidulans AROM sequence has extensive homology with the E. coli aroA gene that encodes the 5-enolpyruvylshikimate 3-phosphate synthase.
摘要
构巢曲霉的AROM基因座控制着分支酸前体芳香族氨基酸生物合成中的五个连续步骤，已被克隆到一个噬菌体载体中。该基因座的核苷酸序列显示有一个4812个碱基对的单一开放阅读框，显然没有内含子。构巢曲霉AROM序列的一个内部片段与编码5-烯醇丙酮酸莽草酸-3-磷酸合酶的大肠杆菌aroA基因有广泛的同源性。
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